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Am J Physiol Heart Circ Physiol 282: H1270-H1277, 2002. First published November 23, 2001; doi:10.1152/ajpheart.00946.2001
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Vol. 282, Issue 4, H1270-H1277, April 2002

Biphasic effects of cell volume on excitation-contraction coupling in rabbit ventricular myocytes

Gui-Rong Li1, Min Zhang2, Leslie S. Satin1,2, and Clive M. Baumgarten1,3

Departments of 1 Physiology, 2 Pharmacology and Toxicology, and 3 Internal Medicine (Cardiology), Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298

We studied the effects of osmotic swelling on the components of excitation-contraction coupling in ventricular myocytes. Myocyte volume rapidly increased 30% in hyposmotic (0.6T) solution and was constant thereafter. Cell shortening transiently increased 31% after 4 min in 0.6T but then decreased to 68% of control after 20 min. In parallel, the L-type Ca2+ current (ICa-L) transiently increased 10% and then declined to 70% of control. Similar biphasic effects on shortening were observed under current clamp. In contrast, action potential duration was unchanged at 4 min but decreased to 72% of control after 20 min. Ca2+ transients were measured with fura 2-AM. The emission ratio with excitation at 340 and 380 nm (f340/f380) decreased by 12% after 3 min in 0.6T, whereas shortening and ICa-L increased at the same time. After 8 min, shortening, ICa-L, and the f340/f380 ratio decreased 28, 25, and 59%, respectively. The results suggest that osmotic swelling causes biphasic changes in ICa-L that contribute to its biphasic effects on contraction. In addition, swelling initially appears to reduce the Ca2+ transient initiated by a given ICa-L, and later, both ICa-L and the Ca2+ transient are inhibited.

osmolarity; action potential duration; calcium current; calcium transient; cell shortening


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