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Am J Physiol Heart Circ Physiol 282: H1656-H1664, 2002. First published January 17, 2002; doi:10.1152/ajpheart.00597.2001
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Vol. 282, Issue 5, H1656-H1664, May 2002

14,15-Dihydroxyeicosatrienoic acid relaxes bovine coronary arteries by activation of KCa channels

William B. Campbell1, Christine Deeter1, Kathryn M. Gauthier1, Richard H. Ingraham2, J. R. Falck3, and Pin-Lan Li1

1 Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226; 2 Boehringer Ingelheim Pharmaceutical, Ridgefield, Connecticut 06877; and 3 Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75482

Epoxyeicosatrienoic acids (EETs) cause vascular relaxation by activating smooth muscle large conductance Ca2+-activated K+ (KCa) channels. EETs are metabolized to dihydroxyeicosatrienoic acids (DHETs) by epoxide hydrolase. We examined the contribution of 14,15-DHET to 14,15-EET-induced relaxations and characterized its mechanism of action. 14,15-DHET relaxed U-46619-precontracted bovine coronary artery rings but was approximately fivefold less potent than 14,15-EET. The relaxations were inhibited by charybdotoxin, iberiotoxin, and increasing extracellular K+ to 20 mM. In isolated smooth muscle cells, 14,15-DHET increased an iberiotoxin-sensitive, outward K+ current and increased KCa channel activity in cell-attached patches and inside-out patches only when GTP was present. 14,15-[14C]EET methyl ester (Me) was converted to 14,15-[14C]DHET-Me, 14,15-[14C]DHET, and 14,15-[14C]EET by coronary arterial rings and endothelial cells but not by smooth muscle cells. The metabolism to 14,15-DHET was inhibited by the epoxide hydrolase inhibitors 4-phenylchalcone oxide (4-PCO) and BIRD-0826. Neither inhibitor altered relaxations to acetylcholine, whereas relaxations to 14,15-EET-Me were increased slightly by BIRD-0826 but not by 4-PCO. 14,15-DHET relaxes coronary arteries through activation of KCa channels. Endothelial cells, but not smooth muscle cells, convert EETs to DHETs, and this conversion results in a loss of vasodilator activity.

endothelium-derived hyperpolarizing factor; epoxyeicosatrienoic acids; potassium channels; epoxide hydrolase


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