| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, Winnipeg, Manitoba, Canada R2H 2A6
We examined the role of the transforming
growth factor (TGF)-
1 signaling inhibitor Smad 7 in
cardiac fibrosis. TGF-1 (10 ng/ml) was found to increase
cytosolic Smad 7 expression in primary adult rat fibroblasts and induce
rapid nuclear export of exogenous Smad 7 in COS-7 cells. Furthermore,
overexpression of Smad 7 in primary adult fibroblasts was associated
with suppressed collagen type I and III expression. We detected Smad 7, phosphorylated Smad 2, TGF- type I receptor (TRI), and
TGF-1 proteins in postmyocardial infarct (MI) rat
hearts. In 2 and 4 wk post-MI hearts, Smad 7 and TRI expression were
decreased in scar tissue, whereas TGF-1 expression was
increased in scar and viable tissue. In the 8 wk post-MI heart, Smad 7 expression was decreased in both scar tissue and myocardium remote to
the infarct scar. Finally, we confirmed that these changes are
paralleled by decreased expression of cytosolic phosphorylated
receptor-regulated Smad 2 in 4-wk viable myocardium and in 2- and 4-wk
infarct scar tissues. Taken together, our data imply that decreased
inhibitory Smad 7 signal in cardiac fibroblasts may play a role in the
pathogenesis of cardiac fibrosis in the post-MI heart.
primary cardiac fibroblasts; transforming growth
factor-1; experimental heart failure; myocardial
infarction
This article has been cited by other articles:
|
|
 |
|
  H. Guo, J. C.K. Leung, M. F. Lam, L. Y.Y. Chan, A. W.L. Tsang, H. Y. Lan, and K. N. Lai Smad7 Transgene Attenuates Peritoneal Fibrosis in Uremic Rats Treated with Peritoneal Dialysis J. Am. Soc. Nephrol., October 1, 2007; 18(10): 2689 - 2703. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Drobic, R. H. Cunnington, K. M. Bedosky, J. E. Raizman, V. V. Elimban, S. G. Rattan, and I. M. C. Dixon Differential and combined effects of cardiotrophin-1 and TGF-beta1 on cardiac myofibroblast proliferation and contraction Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H1053 - H1064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Wang, A. Omar, T. Angelovska, V. Drobic, S. G. Rattan, S. C. Jones, and I. M. C. Dixon Regulation of collagen synthesis by inhibitory Smad7 in cardiac myofibroblasts Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H1282 - H1290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bujak and N. G. Frangogiannis The role of TGF-{beta} signaling in myocardial infarction and cardiac remodeling Cardiovasc Res, May 1, 2007; 74(2): 184 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Leask TGF{beta}, cardiac fibroblasts, and the fibrotic response Cardiovasc Res, May 1, 2007; 74(2): 207 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Gonzalez, F. Le Bellego, and M. S. Ludwig Imbalance of Receptor-Regulated and Inhibitory Smads in Lung Fibroblasts from Bleomycin-Exposed Rats Am. J. Respir. Cell Mol. Biol., February 1, 2007; 36(2): 206 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M.C. Dixon Much ado about bone marrow stem cells: Role in post-myocardial infarct repair Cardiovasc Res, September 1, 2006; 71(4): 609 - 611. [Full Text] [PDF]
|
|
|
|
|
|
G. Euler-Taimor and J. Heger The complex pattern of SMAD signaling in the cardiovascular system Cardiovasc Res, January 1, 2006; 69(1): 15 - 25. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Chatziantoniou and J.-C. Dussaule Insights into the mechanisms of renal fibrosis: is it possible to achieve regression? Am J Physiol Renal Physiol, August 1, 2005; 289(2): F227 - F234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Freed, R. H. Cunnington, A. L. Dangerfield, J. S. Sutton, and I. M.C. Dixon Emerging evidence for the role of cardiotrophin-1 in cardiac repair in the infarcted heart Cardiovasc Res, March 1, 2005; 65(4): 782 - 792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Venkatesan, L. Pini, and M. S. Ludwig Changes in Smad expression and subcellular localization in bleomycin-induced pulmonary fibrosis Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1342 - L1347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rosenkranz TGF-{beta}1 and angiotensin networking in cardiac remodeling Cardiovasc Res, August 15, 2004; 63(3): 423 - 432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Yang, X.-P. Yang, Y.-H. Liu, J. Xu, O. Cingolani, N.-E. Rhaleb, and O. A. Carretero Ac-SDKP Reverses Inflammation and Fibrosis in Rats With Heart Failure After Myocardial Infarction Hypertension, February 1, 2004; 43(2): 229 - 236. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
