AJP - Heart Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 282: H1724-H1731, 2002. First published January 10, 2002; doi:10.1152/ajpheart.00699.2001
0363-6135/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/5/H1724    most recent
00699.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, M.
Right arrow Articles by Roman, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, M.
Right arrow Articles by Roman, R. J.
Vol. 282, Issue 5, H1724-H1731, May 2002

Mechanism of cGMP contribution to the vasodilator response to NO in rat middle cerebral arteries

Ming Yu, Cheng-Wen Sun, Kristopher G. Maier, David R. Harder, and Richard J. Roman

Department of Physiology and Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226

This study examined the mechanism by which cGMP contributes to the vasodilator response to nitric oxide (NO) in rat middle cerebral arteries (MCA). Administration of a NO donor, diethylaminodiazen-1-ium-1,2-dioate (DEA-NONOate), or 8-bromo-cGMP (8-BrcGMP) increased the diameter of serotonin-preconstricted MCA by 79 ± 3%. The response to DEA-NONOate, but not 8-BrcGMP, was attenuated by iberiotoxin (10-7 M) or a 80 mM high-K+ media, suggesting that activation of K+ channels contributes to the vasodilator response to NO but not 8-BrcGMP. The effects of NO and cGMP on the vasoconstrictor response to Ca2+ were also studied in MCA that were permeabilized with alpha -toxin and ionomycin. Elevations in bath Ca2+ from 10-8 to 10-5 M decreased the diameter of permeabilized MCA by 76 ± 5%. DEA-NONOate (10-6 M) and 8-BrcGMP (10-4 M) blunted this response by 60%. Inhibition of guanylyl cyclase with 1H-[1,2,4]oxadiazole[4,3-a] quinoxalin-1-one (10-5 M) blocked the inhibitory effect of the NO donor, but not 8-BrcGMP, on Ca2+-induced vasoconstriction. 8-BrcGMP (10-4 M) had no effect on intracellular Ca2+ concentration ([Ca2+]i) in control, serotonin-stimulated, or alpha -toxin- and ionomycin-permeabilized vascular smooth muscle cells isolated from the MCA. These results indicate that the vasodilator response to NO in rat MCA is mediated by activation of Ca2+-activated K+ channels via a cGMP-independent pathway and that cGMP also contributes to the vasodilator response to NO by decreasing the contractile response to elevations in [Ca2+]i.

vascular smooth muscle; calcium sensitivity; cytochrome P-450


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
E. E. Lloyd, S. P. Marrelli, and R. M. Bryan Jr.
cGMP does not activate two-pore domain K+ channels in cerebrovascular smooth muscle
Am J Physiol Heart Circ Physiol, June 1, 2009; 296(6): H1774 - H1780.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
X. Liu, C. Li, J. R. Falck, R. J. Roman, D. R. Harder, and R. C. Koehler
Interaction of nitric oxide, 20-HETE, and EETs during functional hyperemia in whisker barrel cortex
Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H619 - H631.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Zhu, I. Drenjancevic-Peric, S. McEwen, J. Friesema, D. Schulta, M. Yu, R. J. Roman, and J. H. Lombard
Role of superoxide and angiotensin II suppression in salt-induced changes in endothelial Ca2+ signaling and NO production in rat aorta
Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H929 - H938.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. L. Macdonald, Z.-D. Zhang, M. Takahashi, E. Nikitina, J. Young, A. Xie, and L. Larkin
Calcium sensitivity of vasospastic basilar artery after experimental subarachnoid hemorrhage
Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2329 - H2336.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
K. Takeuchi, N. Miyata, M. Renic, D. R. Harder, and R. J. Roman
Hemoglobin, NO, and 20-HETE interactions in mediating cerebral vasoconstriction following SAH
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R84 - R89.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Yang, J. W. Clark, R. M. Bryan, and C. S. Robertson
Mathematical modeling of the nitric oxide/cGMP pathway in the vascular smooth muscle cell
Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H886 - H897.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. N. Swafford Jr., I. N. Bratz, J. D. Knudson, P. A. Rogers, J. M. Timmerman, J. D. Tune, and G. M. Dick
C-reactive protein does not relax vascular smooth muscle: effects mediated by sodium azide in commercially available preparations
Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1786 - H1795.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Zhu, M. Yu, J. Friesema, T. Huang, R. J. Roman, and J. H. Lombard
Salt-induced ANG II suppression impairs the response of cerebral artery smooth muscle cells to prostacyclin
Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H908 - H913.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. C. Resta
Hypoxic regulation of nitric oxide signaling in vascular smooth muscle
Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L293 - L295.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online