AJP - Heart Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 282: H2066-H2075, 2002. First published February 14, 2002; doi:10.1152/ajpheart.00288.2001
0363-6135/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/6/H2066    most recent
00288.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dai, G.
Right arrow Articles by Kamm, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dai, G.
Right arrow Articles by Kamm, R. D.
Vol. 282, Issue 6, H2066-H2075, June 2002

Endothelial nitric oxide production during in vitro simulation of external limb compression

Guohao Dai1, Olga Tsukurov2, Michael Chen2, Jonathan P. Gertler2, and Roger D. Kamm1

1 Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge 02139; and 2 Vascular Surgery Research Laboratory, Division of Vascular Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114

External pneumatic compression (EPC) is effective in preventing deep vein thrombosis (DVT) and is thought to alter endothelial thromboresistant properties. We investigated the effect of EPC on changes in nitric oxide (NO), a critical mediator in the regulation of vasomotor and platelet function. An in vitro cell culture system was developed to simulate flow and vessel collapse conditions under EPC. Human umbilical vein endothelial cells were cultured and subjected to tube compression (C), pulsatile flow (F), or a combination of the two (FC). NO production and endothelial nitric oxide synthase (eNOS) mRNA expression were measured. The data demonstrate that in the F and FC groups, there is a rapid release of NO followed by a sustained increase. NO production levels in the F and FC groups were almost identical, whereas the C group produced the same low amount of NO as the control group. Conditions F and FC also upregulate eNOS mRNA expression by a factor of 2.08 ± 0.25 and 2.11 ± 0.21, respectively, at 6 h. Experiments with different modes of EPC show that NO production and eNOS mRNA expression respond to different time cycles of compression. These results implicate enhanced NO release as a potentially important factor in the prevention of DVT.

deep vein thrombosis; hemodynamics; nitric oxide synthase


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
A. Uryash, H. Wu, J. Bassuk, P. Kurlansky, M. A. Sackner, and J. A. Adams
Low-amplitude pulses to the circulation through periodic acceleration induces endothelial-dependent vasodilatation
J Appl Physiol, June 1, 2009; 106(6): 1840 - 1847.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
D. A. Kass
Ventricular Arterial Stiffening: Integrating the Pathophysiology
Hypertension, July 1, 2005; 46(1): 185 - 193.
[Abstract] [Full Text] [PDF]

Home page
 PERSPECT VASC SURG ENDOVASC THERHome page
K. T. Delis
The Case for Intermittent Pneumatic Compression of the Lower Extremity as a Novel Treatment in Arterial Claudication
Perspectives in Vascular Surgery and Endovascular Therapy, March 1, 2005; 17(1): 29 - 42.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online