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Am J Physiol Heart Circ Physiol 283: H126-H138, 2002. First published March 14, 2002; doi:10.1152/ajpheart.00622.2001
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Vol. 283, Issue 1, H126-H138, July 2002

Expression and coassociation of ERG1, KCNQ1, and KCNE1 potassium channel proteins in horse heart

Melissa R. Finley1, Yan Li1, Fei Hua2, James Lillich1, Kathy E. Mitchell1, Suhasini Ganta1, Robert F. Gilmour Jr.2, and Lisa C. Freeman1

1 Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas 66506-5802; and 2 Department of Biomedical Sciences, Cornell University, Ithaca, New York 14853-6401

In dogs and in humans, potassium channels formed by ether-a-go-go-related gene 1 protein ERG1 (KCNH2) and KCNQ1 alpha -subunits, in association with KCNE beta -subunits, play a role in normal repolarization and may contribute to abnormal repolarization associated with long QT syndrome (LQTS). The molecular basis of repolarization in horse heart is unknown, although horses exhibit common cardiac arrhythmias and may receive drugs that induce LQTS. In horse heart, we have used immunoblotting and immunostaining to demonstrate the expression of ERG1, KCNQ1, KCNE1, and KCNE3 proteins and RT-PCR to detect KCNE2 message. Peptide N-glycosidase F-sensitive forms of horse ERG1 (145 kDa) and KCNQ1 (75 kDa) were detected. Both ERG1 and KCNQ1 coimmunoprecipitated with KCNE1. Cardiac action potential duration was prolonged by antagonists of either ERG1 (MK-499, cisapride) or KCNQ1/KCNE1 (chromanol 293B). Patch-clamp analysis confirmed the presence of a slow delayed rectifier current. These data suggest that repolarizing currents in horses are similar to those of other species, and that horses are therefore at risk for acquired LQTS. The data also provide unique evidence for coassociation between ERG1 and KCNE1 in cardiac tissue.

KCNH2; KvLQT1; minK; IsK; MiRP; repolarization; equine cardiology


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