AJP - Heart AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 283: H642-H649, 2002. First published April 25, 2002; doi:10.1152/ajpheart.00890.2001
0363-6135/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/2/H642    most recent
00890.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Martin, A. F.
Right arrow Articles by Solaro, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martin, A. F.
Right arrow Articles by Solaro, R. J.
Vol. 283, Issue 2, H642-H649, August 2002

Ca2+ activation and tension cost in myofilaments from mouse hearts ectopically expressing enteric gamma -actin

Anne F. Martin1,*, Ronald M. Phillips1,*, Ajit Kumar2, Kelly Crawford2, Zainab Abbas2, James L. Lessard2, Pieter de Tombe1, and R. John Solaro1

1 Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois 60612; and 2 Children's Hospital Research Foundation, Cincinnati, Ohio 45229

To determine the significance of actin isoforms in chemomechanical coupling, we compared tension and ATPase rate in heart myofilaments from nontransgenic (NTG) and transgenic (TG) mice in which enteric gamma -actin replaced >95% of the cardiac alpha -actin. Enteric gamma -actin was expressed against three backgrounds: mice expressing cardiac alpha -actin, heterozygous null cardiac alpha -actin mice, and homozygous null cardiac alpha -actin mice. There were no differences in maximum Ca2+ activated tension or maximum rate of tension redevelopment after a quick release and rapid restretch protocol between TG and NTG skinned fiber bundles. However, compared with NTG controls, Ca2+ sensitivity of tension was significantly decreased and economy of tension development was significantly increased in myofilaments from all TG hearts. Shifts in myosin isoform population could not fully account for this increase in the economy of force production of TG myofilaments. Our results indicate that an exchange of cardiac alpha -actin with an actin isoform differing in only five amino acids has a significant impact on both Ca2+ regulation of cardiac myofilaments and the cross-bridge cycling rate.

cross bridge; myosin isoforms; energy coupling

* A. F. Martin and R. M. Phillips contributed equally to this study.




This article has been cited by other articles:


Home page
 FASEB J.Home page
M. A. Jaeger, K. J. Sonnemann, D. P. Fitzsimons, K. W. Prins, and J. M. Ervasti
Context-dependent functional substitution of {alpha}-skeletal actin by {gamma}-cytoplasmic actin
FASEB J, July 1, 2009; 23(7): 2205 - 2214.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Rai, C. C. L. Wong, T. Xu, N. A. Leu, D. W. Dong, C. Guo, K. J. McLaughlin, J. R. Yates III, and A. Kashina
Arginyltransferase regulates alpha cardiac actin function, myofibril formation and contractility during heart development
Development, December 1, 2008; 135(23): 3881 - 3889.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. C. G. Daniels, T. Naya, V. L. M. Rundell, and P. P. de Tombe
Development of contractile dysfunction in rat heart failure: hierarchy of cellular events
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R284 - R292.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Thawornkaiwong, J. Pantharanontaga, and J. Wattanapermpool
Hypersensitivity of myofilament response to Ca2+ in association with maladaptation of estrogen-deficient heart under diabetes complication
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R844 - R851.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Clement, M. Stouffs, E. Bettiol, S. Kampf, K.-H. Krause, C. Chaponnier, and M. Jaconi
Expression and function of {alpha}-smooth muscle actin during embryonic-stem-cell-derived cardiomyocyte differentiation
J. Cell Sci., January 15, 2007; 120(2): 229 - 238.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
V. L. M. Rundell, V. Manaves, A. F. Martin, and P. P. de Tombe
Impact of {beta}-myosin heavy chain isoform expression on cross-bridge cycling kinetics
Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H896 - H903.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
V. L. M. Rundell, D. L. Geenen, P. M. Buttrick, and P. P. de Tombe
Depressed cardiac tension cost in experimental diabetes is due to altered myosin heavy chain isoform expression
Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H408 - H413.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. B. Marston and C. S. Redwood
Modulation of Thin Filament Activation by Breakdown or Isoform Switching of Thin Filament Proteins: Physiological and Pathological Implications
Circ. Res., December 12, 2003; 93(12): 1170 - 1178.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online