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Am J Physiol Heart Circ Physiol 283: H1134-H1141, 2002. First published May 2, 2002; doi:10.1152/ajpheart.00065.2002
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Vol. 283, Issue 3, H1134-H1141, September 2002

Platelets activated by transient coronary occlusion exacerbate ischemia-reperfusion injury in rat hearts

Maribel Mirabet1, David Garcia-Dorado1, Javier Inserte1, José A. Barrabés1, Rosa-M. Lidón1, Bernat Soriano1, Marta Azevedo1, Ferran Padilla1, Luis Agulló1, Marisol Ruiz-Meana1, Anna Massaguer2, Pilar Pizcueta2, and Jordi Soler-Soler1

1 Servicio de Cardiología, Hospital Universitari Vall d'Hebron, 08035 Barcelona; 2 Immunology Unit, Department of Cellular Biology and Pathology, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic, 08036 Barcelona, Spain

Platelets (Plt) accumulate in reperfused myocardium but their effect on myocardial necrosis has not been established. We tested the hypothesis that the effect of Plt depends on their activation status. Pig Plt were obtained before 48 min of coronary occlusion (pre-CO-Plt), 10 min after reperfusion (R-Plt), or after a 60-min sham operation (sham-Plt). Plt were infused into isolated rat hearts (n = 124) and subsequently submitted to 60 min of ischemia and 60 min of reperfusion. P-selectin expression was higher (P = 0.02) in R-Plt than in pre-CO-Plt or sham-Plt. Lactate dehydrogenase (LDH) release during reperfusion was similar in hearts receiving pre-CO-Plt, sham-Plt, or no Plt, but R-Plt increased LDH release by 60% (P = 0.004). Activation of pre-CO-Plt with thrombin increased P-selectin expression and LDH release (P < 0.001), and these results were unaffected by tirofiban. There was a close correlation between P-selectin expression and LDH release (r = 0.84; P < 0.001), and myocardial Plt accumulation (r = 0.85; P < 0.001). We conclude that the deleterious effect of Plt on reperfused myocardium depends on their activation status as represented by P-selectin expression, which is enhanced by ischemia-reperfusion.

infarction; myocardial necrosis; myocardial platelet accumulation; P-selectin


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