AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 283: H1446-H1454, 2002. First published June 6, 2002; doi:10.1152/ajpheart.00274.2002
0363-6135/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A corrigendum has been published
Right arrow All Versions of this Article:
283/4/H1446    most recent
00274.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alpert, N. R.
Right arrow Articles by Warshaw, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alpert, N. R.
Right arrow Articles by Warshaw, D. M.
Vol. 283, Issue 4, H1446-H1454, October 2002

Molecular mechanics of mouse cardiac myosin isoforms

Norman R. Alpert1, Christine Brosseau1, Andrea Federico1, Maike Krenz2, Jeffrey Robbins2, and David M. Warshaw1

1 Department of Molecular Physiology and Biophysics, University of Vermont College of Medicine, Burlington, Vermont 05405; and 2 Molecular Cardiovascular Biology, Children's Hospital, Cincinnati, Ohio 45229

Two myosin isoforms are expressed in myocardium, alpha alpha -homodimers (V1) and beta beta -homodimers (V3). V1 exhibits higher velocities and myofibrillar ATPase activities compared with V3. We also observed this for cardiac myosin from normal (V1) and propylthiouracil-treated (V3) mice. Actin velocity in a motility assay (Vactin) over V1 myosin was twice that of V3 as was the myofibrillar ATPase. Myosin's average force (Favg) was similar for V1 and V3. Comparing Vactin and Favg across species for both V1 and V3, our laboratory showed previously (VanBuren P, Harris DE, Alpert NR, and Warshaw DM. Circ Res 77: 439-444, 1995) that mouse V1 has greater Vactin and Favg compared with rabbit V1. Mouse V3 Vactin was twice that of rabbit Vactin. To understand myosin's molecular structure and function, we compared alpha - and beta -cardiac myosin sequences from rodents and rabbits. The rabbit alpha - and beta -cardiac myosin differed by eight and four amino acids, respectively, compared with rodents. These residues are localized to both the motor domain and the rod. These differences in sequence and mechanical performance may be an evolutionary attempt to match a myosin's mechanical behavior to the heart's power requirements.

contractile proteins; heart; motility assay; molecular motor


This article has been cited by other articles:


Home page
 Circ. Res.Home page
C.-K. Du, S. Morimoto, K. Nishii, R. Minakami, M. Ohta, N. Tadano, Q.-W. Lu, Y.-Y. Wang, D.-Y. Zhan, M. Mochizuki, et al.
Knock-In Mouse Model of Dilated Cardiomyopathy Caused by Troponin Mutation
Circ. Res., July 20, 2007; 101(2): 185 - 194.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
B. Schoffstall, N. M. Brunet, S. Williams, V. F. Miller, A. T. Barnes, F. Wang, L. A. Compton, L. A. McFadden, D. W. Taylor, M. Seavy, et al.
Ca2+ sensitivity of regulated cardiac thin filament sliding does not depend on myosin isoform
J. Physiol., December 15, 2006; 577(3): 935 - 944.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
F. S. Korte, T. J. Herron, M. J. Rovetto, and K. S. McDonald
Power output is linearly related to MyHC content in rat skinned myocytes and isolated working hearts
Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H801 - H812.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
N. R. Alpert, S. A. Mohiddin, D. Tripodi, J. Jacobson-Hatzell, K. Vaughn-Whitley, C. Brosseau, D. M. Warshaw, and L. Fananapazir
Molecular and phenotypic effects of heterozygous, homozygous, and compound heterozygote myosin heavy-chain mutations
Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1097 - H1102.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
F. J. Davis, J. B Pillai, M. Gupta, and M. P. Gupta
Concurrent opposite effects of trichostatin A, an inhibitor of histone deacetylases, on expression of {alpha}-MHC and cardiac tubulins: implication for gain in cardiac muscle contractility
Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1477 - H1490.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Krenz, A. Sanbe, F. Bouyer-Dalloz, J. Gulick, R. Klevitsky, T. E. Hewett, H. E. Osinska, J. N. Lorenz, C. Brosseau, A. Federico, et al.
Analysis of Myosin Heavy Chain Functionality in the Heart
J. Biol. Chem., May 2, 2003; 278(19): 17466 - 17474.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online