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Am J Physiol Heart Circ Physiol 283: H1811-H1818, 2002. First published July 11, 2002; doi:10.1152/ajpheart.01092.2001
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Vol. 283, Issue 5, H1811-H1818, November 2002

Presence of cellular renin-angiotensin system in chromaffin cells of bovine adrenal medulla

Jun Ming Wang1,2, Dirk Slembrouck1, Junhui Tan2, Lut Arckens3, Frans H. H. Leenen2, Pierre J. Courtoy4, and Werner P. De Potter1

1 Laboratory of Neuropharmacology and Neurobiology, Department of Medicine, Universitaire Instelling Antwerpen, University of Antwerp, B-2610 Antwerp, Belgium; 2 Hypertension Unit, University of Ottawa Heart Institute, Ottawa, Ontario K1Y 4W7, Canada; 3 Laboratory of Neuroendocrinology and Immunological Biotechnology, Zoological Institute, Katholieke Universiteit Leuven, B-3000 Louvain, Belgium; 4 Cell Biology Unit, Louvain University Medical School, B-1200 Brussels, Belgium

The presence of a local renin-angiotensin system has been established in organs that serve as angiotensin targets. In this study, the expression of angiotensinogen mRNA and subcellular localization of renin, angiotensin-converting enzyme, and angiotensin II were investigated in bovine adrenal medullary cells in primary culture. By light microscopy, expression of angiotensinogen mRNA, immunoreactive renin, angiotensin-converting enzyme, and angiotensin II were readily detectable only in the chromaffin cells. The density distribution of renin and angiotensin II in sucrose gradients suggested a concentration in chromaffin granules, a localization directly confirmed by immunoelectron microscopy. Reverse transcriptase-polymerase chain reaction and sequencing confirmed the expression of angiotensinogen in bovine chromaffin cells and the adrenal medulla. In addition, in vitro autoradiography indicated that both angiotensin-converting enzyme and angiotensin type 1 receptors were present in the adrenal medulla. These results provide the first direct evidence that chromaffin cells in the adrenal medulla are not only the target for angiotensin but should also be considered as potential local angiotensin-generating and -storing cells.

in situ hybridization; immunocytochemistry; radioligand autoradiography; angiotensinogen


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