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cotransporter
Departments of 1 Molecular Genetics, Biochemistry, and Microbiology and 2 Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267
The basolateral
Na+-K+-2Cl
cotransporter (NKCC1)
functions in the maintenance of cellular electrolyte and volume
homeostasis. NKCC1-deficient (Nkcc1/) mice
were used to examine its role in cardiac function and in the
maintenance of blood pressure and vascular tone. Tail-cuff measurements
demonstrated that awake Nkcc1/ mice had
significantly lower systolic blood pressure than wild-type (Nkcc1+/+) mice (114.5 ± 2.2 and
131.8 ± 2.5 mmHg, respectively). Serum aldosterone levels were
normal, indicating that extracellular fluid-volume homeostasis was not
impaired. Studies using pressure transducers in the femoral artery and
left ventricle showed that anesthetized
Nkcc1/ mice have decreased mean arterial
pressure and left ventricular pressure, whereas myocardial contraction
parameters were not significantly different from those of
Nkcc1+/+ mice. When stimulated with
phenylephrine, aortic smooth muscle from
Nkcc1+/+ and Nkcc1/
mice exhibited no significant differences in maximum contractility and
only moderate dose-response shifts. In phasic portal vein smooth muscle
from Nkcc1/ mice, however, a sharp reduction
in mechanical force was noted. These results indicate that NKCC1 can be
important for the maintenance of normal blood pressure and vascular tone.
vasculature; hypotension; bumetanide
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