HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 283/6/H2397    most recent 00371.2001v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (19) Citing Articles via Google Scholar Google Scholar Articles by Wamhoff, B. R. Articles by Sturek, M. Search for Related Content PubMed PubMed Citation Articles by Wamhoff, B. R. Articles by Sturek, M.

Vol. 283, Issue 6, H2397-H2410, December 2002

Exercise training attenuates coronary smooth muscle phenotypic modulation and nuclear Ca²⁺ signaling

B. R. Wamhoff^1,5, D. K. Bowles^3,4, N. J. Dietz^1,4,5, Q. Hu^1,4,5,, and M. Sturek^1,2,4,5

¹ Departments of Physiology and ² Internal Medicine, School of Medicine, ³ Department of Veterinary Biomedical Sciences, School of Veterinary Medicine, ⁴ Dalton Cardiovascular Research Center, and ⁵ Diabetes and Cardiovascular Biology Program, University of Missouri, Columbia, Missouri 65212

Physical inactivity is an independent risk factor for coronary heart disease, yet the mechanism(s) of exercise-related cardioprotection remains unknown. We tested the hypothesis that coronary smooth muscle after exercise training would have decreased mitogen-induced phenotypic modulation and enhanced regulation of nuclear Ca²⁺. Yucatan swine were endurance exercise trained (EX) on a treadmill for 16-20 wk. EX reduced endothelin-1-induced DNA content by 40% compared with sedentary (SED) swine (P < 0.01). EX decreased single cell peak endothelin-1-induced cytosolic Ca²⁺ responses compared with SED by 16% and peak nuclear Ca²⁺ responses by 33% (P < 0.05), as determined by confocal microscopy. On the basis of these results, we hypothesized that sarco(endo)plasmic reticulum Ca²⁺-ATPase (SERCA) and intracellular Ca²⁺ stores in native smooth muscle are spatially localized to dissociate cytosolic Ca²⁺ and nuclear Ca²⁺. Subcellular localization of SERCA in living and fixed cells revealed a distribution of SERCA near the sarcolemma and on the nuclear envelope. These results show that EX enhances nuclear Ca²⁺ regulation, possibly via SERCA, which may be one mechanism by which coronary smooth muscle cells from EX are less responsive to mitogen-induced phenotypic modulation.

endothelin-1; sarco(endo)plasmic reticulum Ca²⁺-ATPase; electron microscopy; fluorescence microscopy; swine

---

Deceased 7 May 2002.

This article has been cited by other articles:

				B. S. Fleenor and D. K. Bowles Exercise training decreases the size and alters the composition of the neointima in a porcine model of percutaneous transluminal coronary angioplasty (PTCA) J Appl Physiol, September 1, 2009; 107(3): 937 - 945. [Abstract] [Full Text] [PDF]

				D. H. J. Thijssen, G. A. Rongen, P. Smits, and M. T. E. Hopman Physical (in)activity and endothelium-derived constricting factors: overlooked adaptations J. Physiol., January 15, 2008; 586(2): 319 - 324. [Abstract] [Full Text] [PDF]

				B. R. Wamhoff, D. K. Bowles, and G. K. Owens Excitation-Transcription Coupling in Arterial Smooth Muscle Circ. Res., April 14, 2006; 98(7): 868 - 878. [Abstract] [Full Text] [PDF]

				A. Pistea, E. N. T. P. Bakker, J. A. E. Spaan, and E. VanBavel Flow inhibits inward remodeling in cannulated porcine small coronary arteries Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2632 - H2640. [Abstract] [Full Text] [PDF]

				E. A. Mokelke, N. J. Dietz, D. M. Eckman, M. T. Nelson, and M. Sturek Diabetic dyslipidemia and exercise affect coronary tone and differential regulation of conduit and microvessel K+ current Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1233 - H1241. [Abstract] [Full Text] [PDF]

				C. A. Witczak and M. Sturek Exercise prevents diabetes-induced impairment in superficial buffer barrier in porcine coronary smooth muscle J Appl Physiol, March 1, 2004; 96(3): 1069 - 1079. [Abstract] [Full Text] [PDF]

				M. A. Thompson, K. K. Henderson, C. R. Woodman, J. R. Turk, J. W. E. Rush, E. Price, and M. H. Laughlin Exercise preserves endothelium-dependent relaxation in coronary arteries of hypercholesterolemic male pigs J Appl Physiol, March 1, 2004; 96(3): 1114 - 1126. [Abstract] [Full Text] [PDF]