AJP - Heart AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 283: H2420-H2430, 2002. First published August 8, 2002; doi:10.1152/ajpheart.00787.2001
0363-6135/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/6/H2420    most recent
00787.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zeng, M.
Right arrow Articles by He, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zeng, M.
Right arrow Articles by He, P.
Vol. 283, Issue 6, H2420-H2430, December 2002

Tumor necrosis factor-alpha -induced leukocyte adhesion and microvessel permeability

Min Zeng2, Hong Zhang3, Clifford Lowell3, and Pingnian He1

1 Department of Physiology and Pharmacology, School of Medicine, West Virginia University, Morgantown, West Virginia 26506-9229; 2 Department of Human Physiology, School of Medicine, University of California, Davis 95616; and 3 Department of Laboratory Medicine, University of California, San Francisco, California 94143-0134

The objective of this study was to investigate whether leukocyte adhesion and/or emigration are critical steps in increased microvessel permeability during acute inflammation. To conduct this study, we combined autologous blood perfusion with a single microvessel perfusion technique, which allows microvessel permeability to be measured precisely after the endothelium has interacted with blood-borne stimuli. Experiments were carried out in intact venular microvessels in rat mesenteries. Firm attachment of leukocytes to endothelial cells was induced by intravenous injection of TNF-alpha (3.5 µg/kg) and resuming autoperfusion in a precannulated microvessel. Leukocyte emigration was facilitated by superfusion of formyl-Met-Leu-Phe-OH. Microvessel permeability was measured as hydraulic conductivity (Lp) or the solute permeability coefficient to tetramethylrhodamine isothiocyanate-labeled alpha -lactalbumin before and after leukocyte adhesion and emigration in individually perfused microvessels. We found that perfusion of a microvessel with TNF-alpha did not affect basal microvessel permeability, but intravenous injection of TNF-alpha caused significant leukocyte adhesion. However, the significant leukocyte adhesion and emigration did not cause corresponding increases in either Lp or solute permeability. Thus our results suggest that leukocyte adhesion and emigration do not necessarily increase microvessel permeability and the mechanisms that regulate the adhesion process act independently from mechanisms that regulate permeability. In addition, silver staining of endothelial boundaries demonstrated that leukocytes preferentially adhere at the junctions of endothelial cells. The appearance of the silver lines indicates that the TNF-alpha -induced firm adhesion of leukocyte to microvessel walls did not involve apparent changes in the junctional structure of endothelial cells, which is consistent with the results of permeability measurements.

hydraulic conductivity; permeability coefficient; leukocyte emigration; silver staining; CD11b/CD18 expression


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Jiang, K. Wen, X. Zhou, D. Schwegler-Berry, V. Castranova, and P. He
Three-dimensional localization and quantification of PAF-induced gap formation in intact venular microvessels
Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H898 - H906.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. P. Sloan, P. A. Shapiro, R. E. DeMeersman, P. S. McKinley, K. J. Tracey, I. Slavov, Y. Fang, and P. D. Flood
Aerobic exercise attenuates inducible TNF production in humans
J Appl Physiol, September 1, 2007; 103(3): 1007 - 1011.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Wang and V. H. Huxley
Adenosine A2A receptor modulation of juvenile female rat skeletal muscle microvessel permeability
Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H3094 - H3105.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. He, H. Zhang, L. Zhu, Y. Jiang, and X. Zhou
Leukocyte-platelet aggregate adhesion and vascular permeability in intact microvessels: role of activated endothelial cells
Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H591 - H599.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
I. H. Sarelius, J. M. Kuebel, J. Wang, and V. H. Huxley
Macromolecule permeability of in situ and excised rodent skeletal muscle arterioles and venules
Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H474 - H480.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. Zhu and P. He
fMLP-stimulated release of reactive oxygen species from adherent leukocytes increases microvessel permeability
Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H365 - H372.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. Zhu, V. Castranova, and P. He
fMLP-stimulated neutrophils increase endothelial [Ca2+]i and microvessel permeability in the absence of adhesion: role of reactive oxygen species
Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1331 - H1338.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. J. Filion and A. S. Popel
Intracoronary administration of FGF-2: a computational model of myocardial deposition and retention
Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H263 - H279.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. K. Shaw, S. Ma, M. B. Kim, R. M. Rao, C. U. Hartman, R. M. Froio, L. Yang, T. Jones, Y. Liu, A. Nusrat, et al.
Coordinated Redistribution of Leukocyte LFA-1 and Endothelial Cell ICAM-1 Accompany Neutrophil Transmigration
J. Exp. Med., December 20, 2004; 200(12): 1571 - 1580.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online