AJP - Heart Calcium Transients and Cell-Sarcomere
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Am J Physiol Heart Circ Physiol 283: H2431-H2439, 2002. First published July 26, 2002; doi:10.1152/ajpheart.00865.2001
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Vol. 283, Issue 6, H2431-H2439, December 2002

Mitochondrial Ca2+ uptake is important over low [Ca2+]i range in arterial smooth muscle

Tomoko Kamishima and John M. Quayle

Department of Human Anatomy and Cell Biology, University of Liverpool, Liverpool L69 3GE, United Kingdom

Mitochondrial Ca2+ uptake is usually thought to occur only when intracellular Ca2+ concentration ([Ca2+]i) is high. We investigated whether mitochondrial Ca2+ removal participates in shaping [Ca2+]i signals in arterial smooth muscle over a low [Ca2+]i range. [Ca2+]i was measured using fura 2-loaded, voltage-clamped cells from rat femoral arteries. Both diazoxide and carbonyl cyanide m-chlorophenylhydrazone (CCCP) depolarized the mitochondria. Diazoxide application increased resting [Ca2+]i, suggesting that Ca2+ is sequestered in mitochondria. Over a low [Ca2+]i range, diazoxide and CCCP slowed Ca2+ removal rate, determined after a brief depolarization. When [Ca2+]i was measured during sustained depolarization to -30 mV, CCCP application increased [Ca2+]i. When Ca2+ transients were repeatedly evoked by caffeine applications, CCCP application elevated resting [Ca2+]i. Caffeine-induced Ca2+ transients were compared before and after CCCP application using the half decay time, or time required to reduce increase in [Ca2+]i by 50% (t1/2). CCCP treatment significantly increased t1/2. These results suggest that Ca2+ removal to mitochondria in arterial smooth muscle cells may be important at a low [Ca2+]i.

carbonyl cyanide m-chlorophenylhydrazone; diazoxide; fura 2; rhodamine 123; sarcoplasmic reticulum


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