AJP - Heart Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 283: H2560-H2566, 2002. First published August 22, 2002; doi:10.1152/ajpheart.00415.2002
0363-6135/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/6/H2560    most recent
00415.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eguchi, D.
Right arrow Articles by Katusic, Z. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eguchi, D.
Right arrow Articles by Katusic, Z. S.
Vol. 283, Issue 6, H2560-H2566, December 2002

Inhibitory effect of recombinant iNOS gene expression on vasomotor function of canine basilar artery

Daihiko Eguchi1, Livius V. d'Uscio1, Chris Wambi1, Deborah Weiler1, Imre Kovesdi2, Timothy O'Brien3, and Zvonimir S. Katusic1

1 Departments of Anesthesiology and Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota 55905; 2 Gene Vec, Incorporated, Gaithersburg, Maryland 20878; and 3 Department of Medicine, Clinical Sciences Institute, University College Hospital, Galway, Ireland

The present study was designed to determine the effect of recombinant inducible nitric oxide (NO) synthase (iNOS) gene expression on vasomotor function in cerebral arteries. Isolated canine basilar arteries were exposed ex vivo (30 min at 37°C) to an adenoviral vector [107, 108, or 109 plaque-forming units (pfu)/ml] encoding either the iNOS gene or the beta -galactosidase reporter gene. Twenty-four hours after transduction, Western blot analysis demonstrated expression of iNOS protein only in iNOS (109 pfu/ml)-transduced arteries. Immunohistochemical analysis localized iNOS expression predominantly in adventitia. Vascular reactivity of isolated basilar arteries was studied by isometric force recording. Concentration-response curves to UTP (10-9-10-3 M) and diethylaminodiazen-1-ium-1,2-dioate (10-10-10-5 M) were significantly shifted to the right in iNOS gene (109 pfu/ml)-transduced rings compared with control and beta -galactosidase-transduced rings (P < 0.05, n = 5-6). Endothelium-dependent relaxation to bradykinin was significantly attenuated in iNOS-transduced rings (P < 0.001, n = 8). The basal level of cGMP and superoxide anion (O<UP><SUB>2</SUB><SUP>−</SUP></UP>·) production were elevated in iNOS-transduced rings (P < 0.05, n = 7 for cGMP; P < 0.01, n = 6-9 for O<UP><SUB>2</SUB><SUP>−</SUP></UP>· production). Our results suggest that expression of recombinant iNOS in cerebral arteries reduces vasomotor reactivity to both vasoconstrictor and vasodilator agonists. Attenuation of contractions is most likely due to functional antagonism between UTP and cGMP. Reduction of endothelium-dependent relaxation to bradykinin appears to be mediated in part by reduced reactivity of smooth muscle cells to NO.

adventitia; bradykinin; nitric oxide; superoxide anion; inducible nitric oxide synthase


This article has been cited by other articles:


Home page
 StrokeHome page
J. Kitayama, F. M. Faraci, C. A. Gunnett, and D. D. Heistad
Impairment of Dilator Responses of Cerebral Arterioles During Diabetes Mellitus: Role of Inducible NO Synthase
Stroke, August 1, 2006; 37(8): 2129 - 2133.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. A. Gunnett, D. D. Lund, F. M. Faraci, and D. D. Heistad
Vascular interleukin-10 protects against LPS-induced vasomotor dysfunction
Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H624 - H630.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
O. Suda, L. A. Smith, L. V. d'Uscio, T. E. Peterson, and Z. S. Katusic
In Vivo Expression of Recombinant Vascular Endothelial Growth Factor in Rabbit Carotid Artery Increases Production of Superoxide Anion
Arterioscler Thromb Vasc Biol, March 1, 2005; 25(3): 506 - 511.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
J. Sorenson, A. V. R. Santhanam, L. A. Smith, M. Akiyama, W. C. Sessa, and Z. S. Katusic
Expression and Function of Recombinant S1179D Endothelial NO Synthase in Human Pial Arteries
Stroke, January 1, 2005; 36(1): 158 - 160.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. G. Chicoine, E. Tzeng, R. Bryan, S. Saenz, M. L. Paffett, J. Jones, C. R. Lyons, T. C. Resta, L. D. Nelin, and B. R. Walker
Intratracheal adenoviral-mediated delivery of iNOS decreases pulmonary vasoconstrictor responses in rats
J Appl Physiol, November 1, 2004; 97(5): 1814 - 1822.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
Z. S. Katusic, N. M. Caplice, and K. A. Nath
Nitric Oxide Synthase Gene Transfer as a Tool to Study Biology of Endothelial Cells
Arterioscler Thromb Vasc Biol, November 1, 2003; 23(11): 1990 - 1994.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online