AJP - Heart Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 284: H185-H192, 2003. First published September 26, 2002; doi:10.1152/ajpheart.00524.2002
0363-6135/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/1/H185    most recent
00524.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (18)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, Y. W.
Right arrow Articles by Toborek, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, Y. W.
Right arrow Articles by Toborek, M.
Vol. 284, Issue 1, H185-H192, January 2003

Redox-regulated mechanisms of IL-4-induced MCP-1 expression in human vascular endothelial cells

Yong Woo Lee1, Bernhard Hennig2, and Michal Toborek1

Departments of 1 Surgery and 2 Animal Sciences, University of Kentucky Medical Center, Lexington, Kentucky 40536

The present study focused on the molecular signaling pathways of monocyte chemoattractant protein-1 (MCP-1) induction by interleukin-4 (IL-4) in human umbilical vein endothelial cells (HUVEC). RT-PCR showed that MCP-1 mRNA accumulation was markedly increased in IL-4-treated HUVEC in a time- and dose-dependent manner. Antioxidants, such as pyrrolidine dithiocarbamate (PDTC) and N-acetylcysteine (NAC), significantly inhibited IL-4-induced MCP-1 mRNA expression. These effects correlated well with the PDTC-mediated inhibition of MCP-1 promoter transcriptional activity observed in IL-4-treated HUVEC. IL-4-induced MCP-1 gene expression was paralleled by a concomitant production of MCP-1 protein. In agreement with MCP-1 gene expression, PDTC attenuated IL-4-mediated induction of MCP-1 protein expression. In addition, IL-4 dramatically increased the transcription factor signal transducers and activators of transcription 1 (STAT1) DNA binding activity, an effect that was attenuated by PDTC. The role of STAT1 in the regulation of the IL-4-induced MCP-1 gene expression was further confirmed in HUVEC transfected with a reporter construct of the MCP-1 promoter with a mutated STAT1 binding site. These results demonstrate that IL-4-dependent MCP-1 induction in HUVEC is mediated by redox-regulated STAT1 activation.

inflammatory cytokine; atherosclerosis; transcriptional regulation; antioxidants; signal transducers and activator of transcription 1; interleukin-4; monocyte chemoattractant protein-1


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
J. H. Boyd, M. Divangahi, L. Yahiaoui, D. Gvozdic, S. Qureshi, and B. J. Petrof
Toll-Like Receptors Differentially Regulate CC and CXC Chemokines in Skeletal Muscle via NF-{kappa}B and Calcineurin
Infect. Immun., December 1, 2006; 74(12): 6829 - 6838.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. Tedgui and Z. Mallat
Cytokines in Atherosclerosis: Pathogenic and Regulatory Pathways
Physiol Rev, April 1, 2006; 86(2): 515 - 581.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
E. Suzuki, H. Satonaka, H. Nishimatsu, S. Oba, R. Takeda, M. Omata, T. Fujita, R. Nagai, and Y. Hirata
Myocyte Enhancer Factor 2 Mediates Vascular Inflammation via the p38-Dependent Pathway
Circ. Res., July 9, 2004; 95(1): 42 - 49.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
Y. W. Lee, S. Y. Eum, A. Nath, and M. Toborek
Estrogen-mediated protection against HIV Tat protein-induced inflammatory pathways in human vascular endothelial cells
Cardiovasc Res, July 1, 2004; 63(1): 139 - 148.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
H. Satonaka, E. Suzuki, H. Nishimatsu, S. Oba, R. Takeda, A. Goto, M. Omata, T. Fujita, R. Nagai, and Y. Hirata
Calcineurin Promotes the Expression of Monocyte Chemoattractant Protein-1 in Vascular Myocytes and Mediates Vascular Inflammation
Circ. Res., March 19, 2004; 94(5): 693 - 700.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online