AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 284: H225-H233, 2003. First published September 26, 2002; doi:10.1152/ajpheart.00698.2002
0363-6135/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/1/H225    most recent
00698.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (27)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, X.-Q.
Right arrow Articles by Cheung, J. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, X.-Q.
Right arrow Articles by Cheung, J. Y.
Vol. 284, Issue 1, H225-H233, January 2003

Phospholemman modulates Na+/Ca2+ exchange in adult rat cardiac myocytes

Xue-Qian Zhang1,*, Anwer Qureshi1,2,*, Jianliang Song1, Lois L. Carl1, Qiang Tian1, Richard C. Stahl1, David J. Carey1, Lawrence I. Rothblum1, and Joseph Y. Cheung1,2

1 Weis Center for Research and 2 Department of Medicine, Geisinger Medical Center, Danville, Pennsylvania 17822

Previous studies have shown that overexpression of phospholemman (PLM) affected contractile function and Ca2+ homeostasis in adult rat myocytes. We tested the hypothesis that PLM modulated Na+/Ca2+ exchanger (NCX1) activity. PLM was overexpressed in adult rat myocytes by adenovirus-mediated gene transfer. After 72 h, the half-time of relaxation from caffeine-induced contracture, an estimate of forward NCX1 activity, was prolonged 1.8-fold (P < 0.003) in myocytes overexpressing PLM compared with control myocytes overexpressing green fluorescent protein alone. Reverse NCX1 current (3 Na+ out:1 Ca2+ in) was significantly (P < 0.0001) lower in PLM myocytes, especially at more positive voltages. Immunofluorescence demonstrated colocalization of PLM and NCX1 to the plasma membrane and t-tubules. Resting membrane potential, action potential amplitude and duration, myocyte size, and NCX1 and calsequestrin protein levels were not affected by PLM overexpression. At 5 mM extracellular [Ca2+] ([Ca2+]o), the depressed contraction amplitudes in PLM myocytes were increased towards normal by cooverexpression with NCX1. At 0.6 mM [Ca2+]o, the supranormal contraction amplitudes in PLM myocytes were reduced by cooverexpression with NCX1. We conclude that PLM modulated myocyte contractility partly by inhibiting Na+/Ca2+ exchange.

primary cardiac myocyte culture; excitation-contraction coupling; edge detection; patch clamp

* X.-Q. Zhang and A. Qureshi contributed equally to this work.




This article has been cited by other articles:


Home page
 CirculationHome page
S. Despa, A. L. Tucker, and D. M. Bers
Phospholemman-Mediated Activation of Na/K-ATPase Limits [Na]i and Inotropic State During {beta}-Adrenergic Stimulation in Mouse Ventricular Myocytes
Circulation, April 8, 2008; 117(14): 1849 - 1855.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. R. Bell, E. Kennington, W. Fuller, K. Dighe, P. Donoghue, J. E. Clark, L.-G. Jia, A. L. Tucker, J. Randall Moorman, M. S. Marber, et al.
Characterization of the phospholemman knockout mouse heart: depressed left ventricular function with increased Na-K-ATPase activity
Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H613 - H621.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
I. Lubarski, S. J. D. Karlish, and H. Garty
Structural and functional interactions between FXYD5 and the Na+-K+-ATPase
Am J Physiol Renal Physiol, December 1, 2007; 293(6): F1818 - F1826.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
F. Han, J. Bossuyt, S. Despa, A. L. Tucker, and D. M. Bers
Phospholemman Phosphorylation Mediates the Protein Kinase C-Dependent Effects on Na+/K+ Pump Function in Cardiac Myocytes
Circ. Res., December 8, 2006; 99(12): 1376 - 1383.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. L. Tucker, J. Song, X.-Q. Zhang, J. Wang, B. A. Ahlers, L. L. Carl, J. P. Mounsey, J. R. Moorman, L. I. Rothblum, and J. Y. Cheung
Altered contractility and [Ca2+]i homeostasis in phospholemman-deficient murine myocytes: role of Na+/Ca2+ exchange
Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2199 - H2209.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. L. Lansbery, L. C. Burcea, M. L. Mendenhall, and R. W. Mercer
Cytoplasmic targeting signals mediate delivery of phospholemman to the plasma membrane
Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1275 - C1286.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Geering
FXYD proteins: new regulators of Na-K-ATPase
Am J Physiol Renal Physiol, February 1, 2006; 290(2): F241 - F250.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
X.-Q. Zhang, J. R. Moorman, B. A. Ahlers, L. L. Carl, D. E. Lake, J. Song, J. P. Mounsey, A. L. Tucker, Y.-m. Chan, L. I. Rothblum, et al.
Phospholemman overexpression inhibits Na+-K+-ATPase in adult rat cardiac myocytes: relevance to decreased Na+ pump activity in postinfarction myocytes
J Appl Physiol, January 1, 2006; 100(1): 212 - 220.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. Reis, L. Zhang, S. Cala, K. N. Jew, L. C. Mace, L. Chung, R. L. Moore, and Y.-C. Ng
Expression of phospholemman and its association with Na+-K+-ATPase in skeletal muscle: effects of aging and exercise training
J Appl Physiol, October 1, 2005; 99(4): 1508 - 1515.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. Despa, J. Bossuyt, F. Han, K. S. Ginsburg, L.-G. Jia, H. Kutchai, A. L. Tucker, and D. M. Bers
Phospholemman-Phosphorylation Mediates the {beta}-Adrenergic Effects on Na/K Pump Function in Cardiac Myocytes
Circ. Res., August 5, 2005; 97(3): 252 - 259.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Song, X.-Q. Zhang, B. A. Ahlers, L. L. Carl, J. Wang, L. I. Rothblum, R. C. Stahl, J. P. Mounsey, A. L. Tucker, J. R. Moorman, et al.
Serine 68 of phospholemman is critical in modulation of contractility, [Ca2+]i transients, and Na+/Ca2+ exchange in adult rat cardiac myocytes
Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2342 - H2354.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L.-G. Jia, C. Donnet, R. C. Bogaev, R. J. Blatt, C. E. McKinney, K. H. Day, S. S. Berr, L. R. Jones, J. R. Moorman, K. J. Sweadner, et al.
Hypertrophy, increased ejection fraction, and reduced Na-K-ATPase activity in phospholemman-deficient mice
Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1982 - H1988.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. A. Mirza, X.-Q. Zhang, B. A. Ahlers, A. Qureshi, L. L. Carl, J. Song, A. L. Tucker, J. P. Mounsey, J. R. Moorman, L. I. Rothblum, et al.
Effects of phospholemman downregulation on contractility and [Ca2+]i transients in adult rat cardiac myocytes
Am J Physiol Heart Circ Physiol, April 1, 2004; 286(4): H1322 - H1330.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. M. Blalock, K.-C. Chen, K. Sharrow, J. P. Herman, N. M. Porter, T. C. Foster, and P. W. Landfield
Gene Microarrays in Hippocampal Aging: Statistical Profiling Identifies Novel Processes Correlated with Cognitive Impairment
J. Neurosci., May 1, 2003; 23(9): 3807 - 3819.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online