AJP - Heart Calcium Transients and Cell-Sarcomere
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 284: H519-H527, 2003. First published September 19, 2002; doi:10.1152/ajpheart.00717.2002
0363-6135/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/2/H519    most recent
00717.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peart, J.
Right arrow Articles by Headrick, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peart, J.
Right arrow Articles by Headrick, J. P.
Vol. 284, Issue 2, H519-H527, February 2003

Receptor and non-receptor-dependent mechanisms of cardioprotection with adenosine

Jason Peart, Laura Willems, and John P. Headrick

Heart Foundation Research Centre, School of Health Science, Griffith University Gold Coast Campus, Southport, Queensland 4217, Australia

The relative roles of mitochondrial (mito) ATP-sensitive K+ (mitoKATP) channels, protein kinase C (PKC), and adenosine kinase (AK) in adenosine-mediated protection were assessed in Langendorff-perfused mouse hearts subjected to 20-min ischemia and 45-min reperfusion. Control hearts recovered 72 ± 3 mmHg of ventricular pressure (50% preischemia) and released 23 ± 2 IU/g lactate dehydrogenase (LDH). Adenosine (50 µM) during ischemia-reperfusion improved recovery (149 ± 8 mmHg) and reduced LDH efflux (5 ± 1 IU/g). Treatment during ischemia alone was less effective. Treatment with 50 µM diazoxide (mitoKATP opener) during ischemia and reperfusion enhanced recovery and was equally effective during ischemia alone. A3 agonism [100 nM 2-chloro-N6-(3-iodobenzyl)-adenosine-5'-N-methyluronamide], A1 agonism (N6-cyclohexyladenosine), and AK inhibition (10 µM iodotubercidin) all reduced necrosis to the same extent as adenosine, but less effectively reduced contractile dysfunction. These responses were abolished by 100 µM 5-hydroxydecanoate (5-HD, mitoKATP channel blocker) or 3 µM chelerythrine (PKC inhibitor). However, the protective effects of adenosine during ischemia-reperfusion were resistant to 5-HD and chelerythrine and only abolished when inhibitors were coinfused with iodotubercidin. Data indicate adenosine-mediated protection via A1/A3 adenosine receptors is mitoKATP channel and PKC dependent, with evidence for a downstream location of PKC. Adenosine provides additional and substantial protection via phosphorylation to 5'-AMP, primarily during reperfusion.

ischemia-reperfusion; mitochondrial adenosine 5'-triphosphate-sensitive K+ channel; mouse; protein kinase C


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Z. Zhu, P. A. Hofmann, and J. K. Buolamwini
Cardioprotective effects of novel tetrahydroisoquinoline analogs of nitrobenzylmercaptopurine riboside in an isolated perfused rat heart model of acute myocardial infarction
Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2921 - H2926.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. Schulze, C. Duschek, R. D. Lasley, and R. Bunger
Adenosine enhances cytosolic phosphorylation potential and ventricular contractility in stunned guinea pig heart: receptor-mediated and metabolic protection
J Appl Physiol, March 1, 2007; 102(3): 1202 - 1213.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
M. E. Reichelt, L. Willems, J. N. Peart, K. J. Ashton, G. P. Matherne, M. R. Blackburn, and J. P. Headrick
Heart/Cardiac Muscle: Modulation of ischaemic contracture in mouse hearts: a 'supraphysiological' response to adenosine
Exp Physiol, January 1, 2007; 92(1): 175 - 185.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
H. Funakoshi, T. O. Chan, J. C. Good, J. R. Libonati, J. Piuhola, X. Chen, S. M. MacDonnell, L. L. Lee, D. E. Herrmann, J. Zhang, et al.
Regulated Overexpression of the A1-Adenosine Receptor in Mice Results in Adverse but Reversible Changes in Cardiac Morphology and Function
Circulation, November 21, 2006; 114(21): 2240 - 2250.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. Turnbull, H.-Z. Zhou, P. M. Swigart, S. Turcato, J. S. Karliner, B. R. Conklin, P. C. Simpson, and A. J. Baker
Sustained preconditioning induced by cardiac transgenesis with the tetracycline transactivator
Am J Physiol Heart Circ Physiol, March 1, 2006; 290(3): H1103 - H1109.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
H. Kin, A. J. Zatta, M. T. Lofye, B. S. Amerson, M. E. Halkos, F. Kerendi, Z.-Q. Zhao, R. A. Guyton, J. P. Headrick, and J. Vinten-Johansen
Postconditioning reduces infarct size via adenosine receptor activation by endogenous adenosine
Cardiovasc Res, July 1, 2005; 67(1): 124 - 133.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
L. Willems, K. J. Ashton, and J. P. Headrick
Adenosine-mediated cardioprotection in the aging myocardium
Cardiovasc Res, May 1, 2005; 66(2): 245 - 255.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. E. Reichelt, L. Willems, J. G. Molina, C.-X. Sun, J. C. Noble, K. J. Ashton, J. Schnermann, M. R. Blackburn, and J. P. Headrick
Genetic Deletion of the A1 Adenosine Receptor Limits Myocardial Ischemic Tolerance
Circ. Res., February 18, 2005; 96(3): 363 - 367.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. P. Headrick, B. Hack, and K. J. Ashton
Acute adenosinergic cardioprotection in ischemic-reperfused hearts
Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H1797 - H1818.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. N. Peart and G. J. Gross
Adenosine and opioid receptor-mediated cardioprotection in the rat: evidence for cross-talk between receptors
Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H81 - H89.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online