AJP - Heart AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 284: H535-H541, 2003. First published October 10, 2002; doi:10.1152/ajpheart.00311.2002
0363-6135/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/2/H535    most recent
00311.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (32)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cerwinka, W. H.
Right arrow Articles by Granger, D. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cerwinka, W. H.
Right arrow Articles by Granger, D. N.
Vol. 284, Issue 2, H535-H541, February 2003

Superoxide mediates endotoxin-induced platelet-endothelial cell adhesion in intestinal venules

Wolfgang H. Cerwinka1, Dianne Cooper1, Christian F. Krieglstein1,4, Chris R. Ross2, Joe M. McCord3, and D. Neil Granger1

1 Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130-3932; 2 Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506-5612; 3 Webb-Waring Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262; and 4 Department of General Surgery, Westphalian Wilhelms University, D48149 Muenster, Germany

Platelets have been implicated in the pathogenesis of different diseases of the vascular system, including atherosclerosis, sepsis, and ischemia-reperfusion injury; however, relatively little is known about the factors that regulate the interactions between circulating platelets and the vessel wall. The objective of this study was to define the contribution of superoxide to LPS-induced platelet-endothelial cell (P/E) adhesion in murine intestinal venules. The adhesion of rhodamine-6G-labeled murine platelets was monitored by intravital fluorescence microscopy. Four hours after LPS administration in control [wild-type (WT)] mice, an ~10-fold increase in P/E adhesion was detected. This response did not result from LPS-induced platelet activation. The LPS-induced P/E adhesion was greatly attenuated in NAD(P)H oxidase-deficient mice and in WT mice rendered neutropenic with anti-neutrophil serum, whereas the response was unchanged in WT mice receiving a CD18 blocking MAb or in CD18-deficient mice. A chimeric form of MnSOD that exhibits the binding properties of extracellular SOD also attenuated the LPS-induced response in WT mice. These findings indicate that neutrophil-derived superoxide plays a major role in the modulation of endotoxin-induced P/E adhesion.

endotoxemia; neutrophils; postcapillary venules


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
K. N. Patel, S. H. Soubra, R. V. Bellera, J.-F. Dong, C. A. McMullen, A. R. Burns, and R. E. Rumbaut
Differential Role of von Willebrand Factor and P-Selectin on Microvascular Thrombosis in Endotoxemia
Arterioscler. Thromb. Vasc. Biol., December 1, 2008; 28(12): 2225 - 2230.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Vowinkel, K. C. Wood, K. Y. Stokes, J. Russell, A. Tailor, C. Anthoni, N. Senninger, C. F. Krieglstein, and D. N. Granger
Mechanisms of platelet and leukocyte recruitment in experimental colitis
Am J Physiol Gastrointest Liver Physiol, November 1, 2007; 293(5): G1054 - G1060.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. E. Rumbaut, R. V. Bellera, J. K. Randhawa, C. N. Shrimpton, S. K. Dasgupta, J.-F. Dong, and A. R. Burns
Endotoxin enhances microvascular thrombosis in mouse cremaster venules via a TLR4-dependent, neutrophil-independent mechanism
Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1671 - H1679.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
T. Petnehazy, K. Y. Stokes, K. C. Wood, J. Russell, and D. N. Granger
Role of Blood Cell-Associated AT1 Receptors in the Microvascular Responses to Hypercholesterolemia
Arterioscler. Thromb. Vasc. Biol., February 1, 2006; 26(2): 313 - 318.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
T. Peng, X. Lu, and Q. Feng
Pivotal Role of gp91phox-Containing NADH Oxidase in Lipopolysaccharide-Induced Tumor Necrosis Factor-{alpha} Expression and Myocardial Depression
Circulation, April 5, 2005; 111(13): 1637 - 1644.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. V. Arumugam, J. W. Salter, J. H. Chidlow, C. M. Ballantyne, C. G. Kevil, and D. N. Granger
Contributions of LFA-1 and Mac-1 to brain injury and microvascular dysfunction induced by transient middle cerebral artery occlusion
Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2555 - H2560.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
F. Krotz, H.-Y. Sohn, and U. Pohl
Reactive Oxygen Species: Players in the Platelet Game
Arterioscler. Thromb. Vasc. Biol., November 1, 2004; 24(11): 1988 - 1996.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. S. Bonder, D. Knight, D. Hernandez-Saavedra, J. M. McCord, and P. Kubes
Chimeric SOD2/3 inhibits at the endothelial-neutrophil interface to limit vascular dysfunction in ischemia-reperfusion
Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G676 - G684.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. C. Wood, R. P. Hebbel, and D. N. Granger
Endothelial cell P-selectin mediates a proinflammatory and prothrombogenic phenotype in cerebral venules of sickle cell transgenic mice
Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1608 - H1614.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. Cooper, J. Russell, K. D. Chitman, M. C. Williams, R. E. Wolf, and D. N. Granger
Leukocyte dependence of platelet adhesion in postcapillary venules
Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1895 - H1900.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Christofidou-Solomidou, A. Scherpereel, R. Wiewrodt, K. Ng, T. Sweitzer, E. Arguiri, V. Shuvaev, C. C. Solomides, S. M. Albelda, and V. R. Muzykantov
PECAM-directed delivery of catalase to endothelium protects against pulmonary vascular oxidative stress
Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L283 - L292.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. Gao, S. C. Flores, J. A. Leff, S. K. Bose, and J. M. McCord
Synthesis and anti-inflammatory activity of a chimeric recombinant superoxide dismutase: SOD2/3
Am J Physiol Lung Cell Mol Physiol, June 1, 2003; 284(6): L917 - L925.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online