AJP - Heart Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 284: H892-H902, 2003. First published November 7, 2002; doi:10.1152/ajpheart.00648.2002
0363-6135/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/3/H892    most recent
00648.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nygren, A.
Right arrow Articles by Giles, W. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nygren, A.
Right arrow Articles by Giles, W. R.
Vol. 284, Issue 3, H892-H902, March 2003

Voltage-sensitive dye mapping in Langendorff-perfused rat hearts

A. Nygren, C. Kondo, R. B. Clark, and W. R. Giles

Department of Physiology and Biophysics, University of Calgary, Calgary, Canada T2N 4N1

An imaging system suitable for recordings from Langendorff-perfused rat hearts using the voltage-sensitive dye 4-[beta -[2-(di-n-butylamino)-6-naphthyl]vinyl]pyridinium (di-4-ANEPPS) has been developed. Conduction velocity was measured under hyper- and hypokalemic conditions, as well as at physiological and reduced temperature. Elevation of extracellular [K+] to 9 mM from 5.9 mM caused a slowing of conduction velocity from 0.66 ± 0.08 to 0.43 ± 0.07 mm/ms (35%), and reduction of the temperature to 32°C from 37°C caused a slowing from 0.64 ± 0.07 to 0.46 ± 0.05 mm/ms (28%). Ventricular activation patterns in sinus rhythm showed areas of early activation (breakthrough) in both the right and left ventricle, with breakthrough at a site near the apex of the right ventricle usually occurring first. The effects of mechanically immobilizing the preparation to reduce motion artifact were also characterized. Activation patterns in epicardially paced rhythm were insensitive to this procedure over the range of applied force tested. In sinus rhythm, however, a relatively large immobilizing force caused prolonged PQ intervals as well as altered ventricular activation patterns. The time-dependent effects of the dye on the rat heart were characterized and include 1) a transient vasodilation at the onset of dye perfusion and 2) a long-lasting prolongation of the PQ interval of the electrocardiogram, frequently resulting in brief episodes of atrioventricular block.

fluorescent dyes; rat heart ventricular activation; imaging techniques


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Weber dos Santos, A. Nygren, F. Otaviano Campos, H. Koch, and W. R. Giles
Experimental and theoretical ventricular electrograms and their relation to electrophysiological gradients in the adult rat heart
Am J Physiol Heart Circ Physiol, October 1, 2009; 297(4): H1521 - H1534.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Shimoni, T. Emmett, R. Schmidt, A. Nygren, and G. Kargacin
Sex-dependent impairment of cardiac action potential conduction in type 1 diabetic rats
Am J Physiol Heart Circ Physiol, May 1, 2009; 296(5): H1442 - H1450.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Rossi, S. Baruffi, A. Bertuzzi, M. Miragoli, D. Corradi, R. Maestri, R. Alinovi, A. Mutti, E. Musso, A. Sgoifo, et al.
Ventricular activation is impaired in aged rat hearts
Am J Physiol Heart Circ Physiol, December 1, 2008; 295(6): H2336 - H2347.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. Hesse, C. S. Kondo, R. B. Clark, L. Su, F. L. Allen, C. T.M. Geary-Joo, S. Kunnathu, D. L. Severson, A. Nygren, W. R. Giles, et al.
Dilated cardiomyopathy is associated with reduced expression of the cardiac sodium channel Scn5a
Cardiovasc Res, August 1, 2007; 75(3): 498 - 509.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. Nygren, M. L. Olson, K. Y. Chen, T. Emmett, G. Kargacin, and Y. Shimoni
Propagation of the cardiac impulse in the diabetic rat heart: reduced conduction reserve
J. Physiol., April 15, 2007; 580(2): 543 - 560.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Matiukas, B. G. Mitrea, A. M. Pertsov, J. P. Wuskell, M.-d. Wei, J. Watras, A. C. Millard, and L. M. Loew
New near-infrared optical probes of cardiac electrical activity
Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2633 - H2643.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
W. R. Mills, N. Mal, F. Forudi, Z. B. Popovic, M. S. Penn, and K. R. Laurita
Optical mapping of late myocardial infarction in rats
Am J Physiol Heart Circ Physiol, March 1, 2006; 290(3): H1298 - H1306.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Nygren, A. E. Lomax, and W. R. Giles
Heterogeneity of action potential durations in isolated mouse left and right atria recorded using voltage-sensitive dye mapping
Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2634 - H2643.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Rubart, E. Wang, K. W. Dunn, and L. J. Field
Two-photon molecular excitation imaging of Ca2+ transients in Langendorff-perfused mouse hearts
Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1654 - C1668.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online