AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 284: H1454-H1459, 2003. First published December 19, 2002; doi:10.1152/ajpheart.00766.2002
0363-6135/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/4/H1454    most recent
00766.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shivakumar, K.
Right arrow Articles by Lakatta, E. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shivakumar, K.
Right arrow Articles by Lakatta, E. G.
Vol. 284, Issue 4, H1454-H1459, April 2003

Differential response of cardiac fibroblasts from young adult and senescent rats to ANG II

K. Shivakumar1, David E. Dostal2, Kenneth Boheler1, Kenneth M. Baker2, and Edward G. Lakatta1

1 Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224; and 2 Division of Molecular Cardiology, The Cardiovascular Research Institute, The Texas A & M University System Health Science Center, Temple, Texas 76504

The intracardiac ANG II-forming pathway is activated in the senescent myocardium, raising the possibility of enhanced ANG II effects on cardiac fibroblasts. This study established an in vitro model of cultured cardiac fibroblasts from aged rats to examine if the response of these cells to ANG II is modified in the aged heart. Levels of mRNA encoding renin, angiotensinogen, and the AT1 receptor subtype in cardiac fibroblasts from young adult and senescent rats were quantified by RT-PCR, net collagen production by a hydroxyproline-based assay, and transforming growth factor (TGF)-beta levels using a commercial kit. In cardiac fibroblasts from young adult rats, ANG II significantly enhanced AT1 mRNA levels, net collagen production, and TGF-beta production. In fibroblasts from the aged myocardium, ANG II downregulated AT1 mRNA expression, had a less pronounced effect on net collagen production, and had no effect on TGF-beta production. Such age-related modification of the response of cardiac fibroblasts to ANG II may counteract the effects of augmented intracardiac ANG II production in the senescent heart, limiting fibrogenesis.

renin-angiotensin system; transforming growth factor-beta ; angiotensin type 1 receptor; collagen; cardiac fibrosis


This article has been cited by other articles:


Home page
 J Am Coll CardiolHome page
M. Bujak, H. J. Kweon, K. Chatila, N. Li, G. Taffet, and N. G. Frangogiannis
Aging-Related Defects Are Associated With Adverse Cardiac Remodeling in a Mouse Model of Reperfused Myocardial Infarction
J. Am. Coll. Cardiol., April 8, 2008; 51(14): 1384 - 1392.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
X. Chen, Z. Li, Z. Feng, J. Wang, C. Ouyang, W. Liu, B. Fu, G. Cai, C. Wu, R. Wei, et al.
Integrin-Linked Kinase Induces Both Senescence-Associated Alterations and Extracellular Fibronectin Assembly in Aging Cardiac Fibroblasts
J. Gerontol. A Biol. Sci. Med. Sci., December 1, 2006; 61(12): 1232 - 1245.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Wang, D. Zhao, G. Spinetti, J. Zhang, L.-Q. Jiang, G. Pintus, R. Monticone, and E. G. Lakatta
Matrix Metalloproteinase 2 Activation of Transforming Growth Factor-{beta}1 (TGF-{beta}1) and TGF-{beta}1-Type II Receptor Signaling Within the Aged Arterial Wall
Arterioscler. Thromb. Vasc. Biol., July 1, 2006; 26(7): 1503 - 1509.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Sapna, S. K. Ranjith, and K. Shivakumar
Cardiac fibrogenesis in magnesium deficiency: a role for circulating angiotensin II and aldosterone
Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H436 - H440.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Wang, H. Chen, A. Seth, and C. A. McCulloch
Mechanical force regulation of myofibroblast differentiation in cardiac fibroblasts
Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H1871 - H1881.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online