| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Physiology and Biophysics, 2 Biomedical Engineering, 3 Medicine, and 4 Biostatistics, University of Alabama, Birmingham, Alabama 35294
We tested whether the interventions typically required for optical mapping affect activation patterns during ventricular fibrillation (VF). A 21 × 24 unipolar electrode array (1.5 mm spacing) was sutured to the left ventricular epicardium of 16 anesthetized pigs, and four episodes of electrically induced VF (30-s duration) were recorded. The hearts were then rapidly excised and connected to a Langendorff perfusion apparatus. Four of the hearts were controls, in which 24 additional VF episodes were then mapped. In the remaining 12 hearts, four VF episodes were mapped after isolation, four more episodes were mapped after exposure to the voltage-sensitive dye di-4-ANEPPS, and six more episodes were mapped after exposure to the electromechanical uncoupling agents diacetyl monoxime (DAM; 20 mmol/l, n = 6) or cytochalasin D (CytoD; 10 µmol/l, n = 6). VF episodes were separated by 4 min. VF activation patterns were quantified using custom pattern analysis algorithms. From comparisons with time-corrected control data, all interventions significantly changed VF patterns. Most changes were broadly consistent with slowing and regularization due to loss of excitability. Heart isolation had the largest effect on VF patterns, followed by CytoD, DAM, and dye.
activation pattern; optical mapping; di-4-ANEPPS; diacetyl monoxime; cytochalasin D
This article has been cited by other articles:
|
|
 |
|
  D. J. Dosdall, P. B. Tabereaux, J. J. Kim, G. P. Walcott, J. M. Rogers, C. R. Killingsworth, J. Huang, P. G. Robertson, W. M. Smith, and R. E. Ideker Chemical ablation of the Purkinje system causes early termination and activation rate slowing of long-duration ventricular fibrillation in dogs Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H883 - H889. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Warren and A. V. Zaitsev Evidence Against the Role of Intracellular Calcium Dynamics in Ventricular Fibrillation Circ. Res., May 9, 2008; 102(9): e103 - e103. [Full Text] [PDF]
|
|
|
|
 |
|
 P. B. Tabereaux, G. P. Walcott, J. M. Rogers, J. Kim, D. J. Dosdall, P. G. Robertson, C. R. Killingsworth, W. M. Smith, and R. E. Ideker Activation Patterns of Purkinje Fibers During Long-Duration Ventricular Fibrillation in an Isolated Canine Heart Model Circulation, September 4, 2007; 116(10): 1113 - 1119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nanthakumar, J. Jalife, S. Masse, E. Downar, M. Pop, J. Asta, H. Ross, V. Rao, S. Mironov, E. Sevaptsidis, et al. Optical mapping of Langendorff-perfused human hearts: establishing a model for the study of ventricular fibrillation in humans Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H875 - H880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Kay, G. P. Walcott, J. D. Gladden, S. B. Melnick, and J. M. Rogers Lifetimes of epicardial rotors in panoramic optical maps of fibrillating swine ventricles Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1935 - H1941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-R. Kong, N. Bursac, and L. Tung Mechanoelectrical excitation by fluid jets in monolayers of cultured cardiac myocytes J Appl Physiol, June 1, 2005; 98(6): 2328 - 2336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Cheng, L. Li, V. Nikolski, D. W. Wallick, and I. R. Efimov Shock-induced arrhythmogenesis is enhanced by 2,3-butanedione monoxime compared with cytochalasin D Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H310 - H318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Chattipakorn, I. Banville, R. A Gray, and R. E Ideker Effects of shock strengths on ventricular defibrillation failure Cardiovasc Res, January 1, 2004; 61(1): 39 - 44. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
