AJP - Heart Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 284: H2106-H2113, 2003. First published February 21, 2003; doi:10.1152/ajpheart.00057.2003
0363-6135/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/6/H2106    most recent
00057.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (47)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gumina, R. J.
Right arrow Articles by Terzic, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gumina, R. J.
Right arrow Articles by Terzic, A.
Vol. 284, Issue 6, H2106-H2113, June 2003

Knockout of Kir6.2 negates ischemic preconditioning-induced protection of myocardial energetics

Richard J. Gumina1, Darko Pucar1, Peter Bast1, Denice M. Hodgson1, Christopher E. Kurtz1, Petras P. Dzeja1, Takashi Miki2, Susumu Seino2, and Andre Terzic1

1 Departments of Internal Medicine and Molecular Pharmacology and Experimental Therapeutics, Division of Cardiovascular Diseases, Mayo Clinic, Mayo Foundation, Rochester, Minnesota 55905; and 2 Department of Cellular and Molecular Medicine, Chiba University Graduate School of Medicine, Chiba-shi 260-8670, Japan

Although ischemic preconditioning induces bioenergetic tolerance and thereby remodels energy metabolism that is crucial for postischemic recovery of the heart, the molecular components associated with preservation of cellular energy production, transfer, and utilization are not fully understood. Here myocardial bioenergetic dynamics were assessed by 18O-assisted 31P-NMR spectroscopy in control or preconditioned hearts from wild-type (WT) or Kir6.2-knockout (Kir6.2-KO) mice that lack metabolism-sensing sarcolemmal ATP-sensitive K+ (KATP) channels. In WT vs. Kir6.2-KO hearts, preconditioning induced a significantly higher total ATP turnover (232 ± 20 vs. 155 ± 15 nmol · mg protein-1 · min-1), ATP synthesis rate (58 ± 3 vs. 46 ± 3% 18O labeling of gamma -ATP), and ATP consumption rate (51 ± 4 vs. 31 ± 4% 18O labeling of Pi) after ischemia-reperfusion. Moreover, preconditioning preserved cardiac creatine kinase-catalyzed phosphotransfer in WT (234 ± 26 nmol · mg protein-1 · min-1) but not Kir6.2-KO (133 ± 18 nmol · mg protein-1 · min-1) hearts. In contrast with WT hearts, preconditioning failed to preserve contractile recovery in Kir6.2-KO hearts, as tight coupling between postischemic performance and high-energy phosphoryl transfer was compromised in the KATP-channel-deficient myocardium. Thus intact KATP channels are integral in ischemic preconditioning-induced protection of cellular energetic dynamics and associated cardiac performance.

ATP-sensitive K+ channel; cardioprotection; ischemia; metabolism


This article has been cited by other articles:


Home page
 CirculationHome page
J. W. Elrod, M. Harrell, T. P. Flagg, S. Gundewar, M. A. Magnuson, C. G. Nichols, W. A. Coetzee, and D. J. Lefer
Role of Sulfonylurea Receptor Type 1 Subunits of ATP-Sensitive Potassium Channels in Myocardial Ischemia/Reperfusion Injury
Circulation, March 18, 2008; 117(11): 1405 - 1413.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. A. Burke, R. K. Mutharasan, and H. Ardehali
The Sulfonylurea Receptor, an Atypical ATP-Binding Cassette Protein, and Its Regulation of the KATP Channel
Circ. Res., February 1, 2008; 102(2): 164 - 176.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
J.-D. Jiao, V. Garg, B. Yang, and K. Hu
Novel functional role of heat shock protein 90 in ATP-sensitive K+ channel-mediated hypoxic preconditioning
Cardiovasc Res, January 1, 2008; 77(1): 126 - 133.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. Jahangir, S. Sagar, and A. Terzic
Aging and cardioprotection
J Appl Physiol, December 1, 2007; 103(6): 2120 - 2128.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. A. Brown and R. L. Moore
Perspectives in innate and acquired cardioprotection: cardioprotection acquired through exercise
J Appl Physiol, November 1, 2007; 103(5): 1894 - 1899.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. V. Zingman, A. E. Alekseev, D. M. Hodgson-Zingman, and A. Terzic
ATP-sensitive potassium channels: metabolic sensing and cardioprotection
J Appl Physiol, November 1, 2007; 103(5): 1888 - 1893.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
V. Garg and K. Hu
Protein kinase C isoform-dependent modulation of ATP-sensitive K+ channels in mitochondrial inner membrane
Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H322 - H332.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. J. Chicco, M. S. Johnson, C. J. Armstrong, J. M. Lynch, R. T. Gardner, G. S. Fasen, C. P. Gillenwater, and R. L. Moore
Sex-specific and exercise-acquired cardioprotection is abolished by sarcolemmal KATP channel blockade in the rat heart
Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2432 - H2437.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. J. Gumina, D. F. O'Cochlain, C. E. Kurtz, P. Bast, D. Pucar, P. Mishra, T. Miki, S. Seino, S. Macura, and A. Terzic
KATP channel knockout worsens myocardial calcium stress load in vivo and impairs recovery in stunned heart
Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1706 - H1713.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. L. Moore
Myocardial KATP channels are critical to Ca2+ homeostasis in the metabolically stressed heart in vivo
Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1692 - H1693.
[Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Yamada, G. C. Kane, A. Behfar, X.-K. Liu, R. B. Dyer, R. S. Faustino, T. Miki, S. Seino, and A. Terzic
Protection conferred by myocardial ATP-sensitive K+ channels in pressure overload-induced congestive heart failure revealed in KCNJ11 Kir6.2-null mutant
J. Physiol., December 15, 2006; 577(3): 1053 - 1065.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
K. Fang, L. Csanady, and K. W. Chan
The N-terminal transmembrane domain (TMD0) and a cytosolic linker (L0) of sulphonylurea receptor define the unique intrinsic gating of KATP channels
J. Physiol., October 15, 2006; 576(2): 379 - 389.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
X. Tong, L. M. Porter, G. Liu, P. Dhar-Chowdhury, S. Srivastava, D. J. Pountney, H. Yoshida, M. Artman, G. I. Fishman, C. Yu, et al.
Consequences of cardiac myocyte-specific ablation of KATP channels in transgenic mice expressing dominant negative Kir6 subunits
Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H543 - H551.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
H.-S. Sun, Z.-P. Feng, T. Miki, S. Seino, and R. J. French
Enhanced Neuronal Damage After Ischemic Insults in Mice Lacking Kir6.2-Containing ATP-Sensitive K+ Channels
J Neurophysiol, April 1, 2006; 95(4): 2590 - 2601.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
R. Kakkar, B. Ye, D. A. Stoller, M. Smelley, N.-Q. Shi, K. Galles, M. Hadhazy, J. C. Makielski, and E. M. McNally
Spontaneous Coronary Vasospasm in KATP Mutant Mice Arises From a Smooth Muscle-Extrinsic Process
Circ. Res., March 17, 2006; 98(5): 682 - 689.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
P. Tammaro, P. Proks, and F. M. Ashcroft
Functional effects of naturally occurring KCNJ11 mutations causing neonatal diabetes on cloned cardiac KATP channels
J. Physiol., February 15, 2006; 571(1): 3 - 14.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D. A. Brown, A. J. Chicco, K. N. Jew, M. S. Johnson, J. M. Lynch, P. A. Watson, and R. L. Moore
Cardioprotection afforded by chronic exercise is mediated by the sarcolemmal, and not the mitochondrial, isoform of the KATP channel in the rat
J. Physiol., December 15, 2005; 569(3): 913 - 924.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D. A Brown, J. M Lynch, C. J Armstrong, N. M Caruso, L. B Ehlers, M. S Johnson, and R. L Moore
Susceptibility of the heart to ischaemia-reperfusion injury and exercise-induced cardioprotection are sex-dependent in the rat
J. Physiol., April 15, 2005; 564(2): 619 - 630.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. H. Patel, E. R. Gross, J. N. Peart, A. K. Hsu, and G. J. Gross
Sarcolemmal KATP channel triggers delayed ischemic preconditioning in rats
Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H445 - H447.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
R. J. Diaz, C. Zobel, H. Cheol Cho, M. Batthish, A. Hinek, P. H. Backx, and G. J. Wilson
Selective Inhibition of Inward Rectifier K+ Channels (Kir2.1 or Kir2.2) Abolishes Protection by Ischemic Preconditioning in Rabbit Ventricular Cardiomyocytes
Circ. Res., August 6, 2004; 95(3): 325 - 332.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
B. O'Rourke
Evidence for Mitochondrial K+ Channels and Their Role in Cardioprotection
Circ. Res., March 5, 2004; 94(4): 420 - 432.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.