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Am J Physiol Heart Circ Physiol 284: H2213-H2226, 2003. First published February 13, 2003; doi:10.1152/ajpheart.00999.2002
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Vol. 284, Issue 6, H2213-H2226, June 2003

Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node

Mitchell H. Grayson1, Richard S. Hotchkiss4,6, Irene E. Karl2, Michael J. Holtzman3,5, and David D. Chaplin7

Divisions of 1 Allergy and Immunology, 2 Metabolism, and 3 Pulmonary and Critical Care Medicine, 4 Department of Medicine, and Departments of 5 Cell Biology and 6 Anesthesiology, Washington University School of Medicine, St. Louis, Missouri 63110; and 7 Department of Microbiology, University of Alabama, Birmingham, Alabama 35294

Lymphocyte rolling velocity is determined largely by interactions between leukocyte alpha 4-integrin (CD49d) and L-selectin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in mesenteric postcapillary venules and Peyer's patch high endothelial venules (HEVs). The role of these interactions in other tissue sites of lymphocyte emigration is not known. With the use of real-time intravital confocal microscopy, we found that rolling velocities of T lymphocytes in the murine mesenteric lymph node (MLN) HEV also depend on L-selectin and CD49d. However, in the murine spleen, rolling velocities of T lymphocytes are not influenced by the loss of L-selectin and CD49d. With the use of FITC-dextran and TIE2-GFP mice, we further defined the microvascular compartments of the spleen and showed that adherence of T cells is localized to regions in the white pulp that are not lined by endothelial cells and have shear rates similar to bone marrow sinusoids. These results establish that T cell trafficking to the spleen differs from trafficking to other secondary lymphoid organs and suggest that the mechanical properties of the blood-filtering role of the spleen are important in T cell accumulation in the organ.

cell adhesion molecule; lymphocyte rolling; hemodynamic parameters; microvascular vessels


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