AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 284: H2213-H2226, 2003. First published February 13, 2003; doi:10.1152/ajpheart.00999.2002
0363-6135/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Videos
Right arrow All Versions of this Article:
284/6/H2213    most recent
00999.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grayson, M. H.
Right arrow Articles by Chaplin, D. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grayson, M. H.
Right arrow Articles by Chaplin, D. D.
Vol. 284, Issue 6, H2213-H2226, June 2003

Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node

Mitchell H. Grayson1, Richard S. Hotchkiss4,6, Irene E. Karl2, Michael J. Holtzman3,5, and David D. Chaplin7

Divisions of 1 Allergy and Immunology, 2 Metabolism, and 3 Pulmonary and Critical Care Medicine, 4 Department of Medicine, and Departments of 5 Cell Biology and 6 Anesthesiology, Washington University School of Medicine, St. Louis, Missouri 63110; and 7 Department of Microbiology, University of Alabama, Birmingham, Alabama 35294

Lymphocyte rolling velocity is determined largely by interactions between leukocyte alpha 4-integrin (CD49d) and L-selectin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in mesenteric postcapillary venules and Peyer's patch high endothelial venules (HEVs). The role of these interactions in other tissue sites of lymphocyte emigration is not known. With the use of real-time intravital confocal microscopy, we found that rolling velocities of T lymphocytes in the murine mesenteric lymph node (MLN) HEV also depend on L-selectin and CD49d. However, in the murine spleen, rolling velocities of T lymphocytes are not influenced by the loss of L-selectin and CD49d. With the use of FITC-dextran and TIE2-GFP mice, we further defined the microvascular compartments of the spleen and showed that adherence of T cells is localized to regions in the white pulp that are not lined by endothelial cells and have shear rates similar to bone marrow sinusoids. These results establish that T cell trafficking to the spleen differs from trafficking to other secondary lymphoid organs and suggest that the mechanical properties of the blood-filtering role of the spleen are important in T cell accumulation in the organ.

cell adhesion molecule; lymphocyte rolling; hemodynamic parameters; microvascular vessels


This article has been cited by other articles:


Home page
 BloodHome page
S. Yakubenia, D. Frommhold, D. Scholch, C. C. Hellbusch, C. Korner, B. Petri, C. Jones, U. Ipe, M. G. Bixel, R. Krempien, et al.
Leukocyte trafficking in a mouse model for leukocyte adhesion deficiency II/congenital disorder of glycosylation IIc
Blood, August 15, 2008; 112(4): 1472 - 1481.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
M. H. Grayson, D. Cheung, M. M. Rohlfing, R. Kitchens, D. E. Spiegel, J. Tucker, J. T. Battaile, Y. Alevy, L. Yan, E. Agapov, et al.
Induction of high-affinity IgE receptor on lung dendritic cells during viral infection leads to mucous cell metaplasia
J. Exp. Med., October 29, 2007; 204(11): 2759 - 2769.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.