| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Cardiac Research Laboratory, Department of Cardiothoracic Surgery, and 2 Neurosciences Laboratory, Felsenstein Medical Research Center, Rabin Medical Center, Tel Aviv University, Tel Aviv 69978; 3 Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 52900, Israel; and 4 Cardiovascular Research Center, University of Connecticut School of Medicine, Farmington, Connecticut 06030-1110
The
role of the proapototic Bax gene in ischemia-reperfusion (I/R)
injury was studied in three groups of mice: homozygotic knockout mice
lacking the Bax gene (Bax
/), heterozygotic mice
(Bax+/), and wild-type mice (Bax+/+).
Isolated hearts were subjected to ischemia (30 min, 37°C) and then to 120 min of reperfusion. The left ventricular developed force of
Bax-deficient vs. Bax+/+ hearts at stabilization and at 120 min of reperfusion was 1,411 ± 177 vs. 1,161 ± 137 mg and
485 ± 69 vs. 306 ± 68 mg, respectively. Superior cardiac
function of Bax/ hearts after I/R was accompanied by a
decrease in creatine kinase release, caspase 3 activity, irreversible
ischemic injury, and the number of terminal deoxynucleotidyl
transferase-mediated dUTP nick-end labeling-positive cardiomyocytes.
Electron microscopic evaluation revealed reduced damage to mitochondria
and the nuclear chromatin structure in Bax-deficient mice. In the
Bax+/ hearts, the damage markers were moderate. The
superior tolerance of Bax knockout hearts to I/R injury recommends this
gene as a potential target for therapeutic intervention in patients
with severe and intractable myocardial ischemia.
Bax-deficient hearts; heart; apoptosis
This article has been cited by other articles:
|
|
 |
|
  S. Miyamoto, M. Rubio, and M. A. Sussman Nuclear and mitochondrial signalling Akts in cardiomyocytes Cardiovasc Res, May 1, 2009; 82(2): 272 - 285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Gustafsson and R. A. Gottlieb Autophagy in Ischemic Heart Disease Circ. Res., January 30, 2009; 104(2): 150 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ramachandran, S. Jha, and D. J. Lefer REVIEW Paper: Pathophysiology of Myocardial Reperfusion Injury: The Role of Genetically Engineered Mouse Models Vet. Pathol., September 1, 2008; 45(5): 698 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-L. Yue, S. S. Nerurkar, W. Bao, B. M. Jucker, L. Sarov-Blat, K. Steplewski, E. H. Ohlstein, and R. N. Willette In Vivo Activation of Peroxisome Proliferator-Activated Receptor-{delta} Protects the Heart from Ischemia/Reperfusion Injury in Zucker Fatty Rats J. Pharmacol. Exp. Ther., May 1, 2008; 325(2): 466 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Murphy and C. Steenbergen Mechanisms Underlying Acute Protection From Cardiac Ischemia-Reperfusion Injury Physiol Rev, April 1, 2008; 88(2): 581 - 609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Moresi, A. Pristera, B. M. Scicchitano, M. Molinaro, L. Teodori, D. Sassoon, S. Adamo, and D. Coletti Tumor Necrosis Factor-{alpha} Inhibition of Skeletal Muscle Regeneration Is Mediated by a Caspase-Dependent Stem Cell Response Stem Cells, April 1, 2008; 26(4): 997 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Gustafsson and R. A. Gottlieb Heart mitochondria: gates of life and death Cardiovasc Res, January 15, 2008; 77(2): 334 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Elmore Apoptosis: A Review of Programmed Cell Death Toxicol Pathol, June 1, 2007; 35(4): 495 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Gustafsson and R. A. Gottlieb Bcl-2 family members and apoptosis, taken to heart Am J Physiol Cell Physiol, January 1, 2007; 292(1): C45 - C51. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Bahi, J. Zhang, M. Llovera, M. Ballester, J. X. Comella, and D. Sanchis Switch from Caspase-dependent to Caspase-independent Death during Heart Development: ESSENTIAL ROLE OF ENDONUCLEASE G IN ISCHEMIA-INDUCED DNA PROCESSING OF DIFFERENTIATED CARDIOMYOCYTES J. Biol. Chem., August 11, 2006; 281(32): 22943 - 22952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Toth, J. R. Jeffers, P. Nickson, J.-Y. Min, J. P. Morgan, G. P. Zambetti, and P. Erhardt Targeted deletion of Puma attenuates cardiomyocyte death and improves cardiac function during ischemia-reperfusion Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H52 - H60. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Khan, S. Varadharaj, L. P. Ganesan, J. C. Shobha, M. U. Naidu, N. L. Parinandi, S. Tridandapani, V. K. Kutala, and P. Kuppusamy C-phycocyanin protects against ischemia-reperfusion injury of heart through involvement of p38 MAPK and ERK signaling Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H2136 - H2145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Hou and Y.-T. Hsu Bax translocates from cytosol to mitochondria in cardiac cells during apoptosis: development of a GFP-Bax-stable H9c2 cell line for apoptosis analysis Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H477 - H487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Crow, K. Mani, Y.-J. Nam, and R. N. Kitsis The Mitochondrial Death Pathway and Cardiac Myocyte Apoptosis Circ. Res., November 12, 2004; 95(10): 957 - 970. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
