HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/1/H292    most recent 01059.2002v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by Leffler, C. W. Articles by Parfenova, H. Search for Related Content PubMed PubMed Citation Articles by Leffler, C. W. Articles by Parfenova, H.

Regulation of CO production in cerebral microvessels of newborn pigs

Laboratory for Research in Neonatal Physiology, Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163

Submitted 9 December 2002 ; accepted in final form 3 March 2003

Carbon monoxide (CO) is produced from heme by heme oxygenase-2 (HO-2) in cerebral blood vessels. Gas chromatography-mass spectrometry was used on piglet cerebral microvessels to address the hypothesis that CO production is regulated by heme delivery and HO-2 catalytic activity. CO production appears to be substrate limited because heme and its precursor aminolevulinate increase CO production. Ionomycin also increases CO production. However, CO production from exogenous heme was the same in Ca-replete medium, Ca-free medium with ionomycin, and Ca-replete medium with ionomycin. Phorbol myristate acetate increases CO production but does not change the catalytic activity of HO-2. Also, the protein kinase C inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine has no effect on the HO-2 catalytic activity. Protein tyrosine kinase inhibition reduces HO-2 catalytic activity. Inhibition of protein tyrosine phosphatases increased HO-2 catalytic activity. Therefore, regulation of CO production by cerebral microvessels can include changing heme availability and HO-2 catalytic activity. HO-2 catalytic activity is stimulated by tyrosine phosphorylation.

heme oxygenase; cerebrovascular; phosphorylation; calcium; carbon monoxide

This article has been cited by other articles:

				A. Kanu and C. W. Leffler Roles of Glia Limitans Astrocytes and Carbon Monoxide in Adenosine Diphosphate-Induced Pial Arteriolar Dilation in Newborn Pigs Stroke, March 1, 2009; 40(3): 930 - 935. [Abstract] [Full Text] [PDF]

				C. W. Leffler, H. Parfenova, A. L. Fedinec, S. Basuroy, and D. Tcheranova Contributions of astrocytes and CO to pial arteriolar dilation to glutamate in newborn pigs Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H2897 - H2904. [Abstract] [Full Text] [PDF]

				S. Basuroy, S. Bhattacharya, D. Tcheranova, Y. Qu, R. F. Regan, C. W. Leffler, and H. Parfenova HO-2 provides endogenous protection against oxidative stress and apoptosis caused by TNF-{alpha} in cerebral vascular endothelial cells Am J Physiol Cell Physiol, November 1, 2006; 291(5): C897 - C908. [Abstract] [Full Text] [PDF]

				A. Kanu, J. Whitfield, and C. W. Leffler Carbon monoxide contributes to hypotension-induced cerebrovascular vasodilation in piglets Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2409 - H2414. [Abstract] [Full Text] [PDF]

				C. W. Leffler, H. Parfenova, J. H. Jaggar, and R. Wang Carbon monoxide and hydrogen sulfide: gaseous messengers in cerebrovascular circulation J Appl Physiol, March 1, 2006; 100(3): 1065 - 1076. [Abstract] [Full Text] [PDF]

				A. Rebel, S. Cao, H. Kwansa, S. Dore, E. Bucci, and R. C. Koehler Dependence of acetylcholine and ADP dilation of pial arterioles on heme oxygenase after transfusion of cell-free polymeric hemoglobin Am J Physiol Heart Circ Physiol, March 1, 2006; 290(3): H1027 - H1037. [Abstract] [Full Text] [PDF]

				A. Kanu, D. Gilpin, A. L. Fedinec, and C. W. Leffler Cyclooxygenase Products Stimulate Carbon Monoxide Production by Piglet Cerebral Microvessels Experimental Biology and Medicine, February 1, 2006; 231(2): 181 - 185. [Abstract] [Full Text] [PDF]

				C. W. Leffler, L. Balabanova, A. L. Fedinec, and H. Parfenova Nitric oxide increases carbon monoxide production by piglet cerebral microvessels Am J Physiol Heart Circ Physiol, October 1, 2005; 289(4): H1442 - H1447. [Abstract] [Full Text] [PDF]

				E. M. Sanhueza, R. A. Riquelme, E. A. Herrera, D. A. Giussani, C. E. Blanco, M. A. Hanson, and A. J. Llanos Vasodilator tone in the llama fetus: the role of nitric oxide during normoxemia and hypoxemia Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2005; 289(3): R776 - R783. [Abstract] [Full Text] [PDF]

				P. Li, H. Jiang, L. Yang, S. Quan, S. Dinocca, F. Rodriguez, N. G. Abraham, and A. Nasjletti Angiotensin II induces carbon monoxide production in the perfused kidney: relationship to protein kinase C activation Am J Physiol Renal Physiol, November 1, 2004; 287(5): F914 - F920. [Abstract] [Full Text] [PDF]

				Q. Xi, D. Tcheranova, H. Parfenova, B. Horowitz, C. W. Leffler, and J. H. Jaggar Carbon monoxide activates KCa channels in newborn arteriole smooth muscle cells by increasing apparent Ca2+ sensitivity of {alpha}-subunits Am J Physiol Heart Circ Physiol, February 1, 2004; 286(2): H610 - H618. [Abstract] [Full Text] [PDF]