HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/1/H298    most recent 00043.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (18) Citing Articles via Google Scholar Google Scholar Articles by Yang, G. Articles by Iadecola, C. Search for Related Content PubMed PubMed Citation Articles by Yang, G. Articles by Iadecola, C.

Attenuation of activity-induced increases in cerebellar blood flow in mice lacking neuronal nitric oxide synthase

Division of Neurobiology, Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, New York 10021

Submitted 16 January 2003 ; accepted in final form 4 March 2003

We used mice deficient in neuronal nitric oxide (NO) synthase (nNOS) to specifically investigate the role of neuronal NO in the increase of cerebellar blood flow (BF_crb) produced by neural activation. Crus II, a region of the cerebellar cortex that receives trigeminal sensory afferents, was activated by low-intensity stimulation of the upper lip (5–25 V, 4–16 Hz) in anesthetized mice. BF_crb was recorded in Crus II by using a laser-Doppler flow probe. In wild-type mice, upper lip stimulation increased BF_crb in the Crus II by 28 ± 3% (25 V, 10 Hz, n = 6). The rise in BF_crb was attenuated by 73 ± 3% in nNOS^-/- mice (P < 0.05, n = 6). The increases in BF_crb produced by superfusion of Crus II with glutamate or by systemic administration of harmaline were also attenuated in nNOS^-/- mice (P < 0.05). In contrast, the increases in BF_crb produced by topical superfusion of Crus II with acetylcholine or adenosine and the increase in BF_crb produced by hypercapnia were not affected (P > 0.05). The field potentials evoked in the Crus II by upper lip stimulation did not differ between wild-type and nNOS-null mice. These data provide the first nonpharmacological evidence that nNOS-derived NO is a critical link between glutamatergic synaptic activity and blood flow in the activated cerebellum.

cerebral circulation; cerebellum; laser-Doppler flowmetry; vasodilation; glutamate

This article has been cited by other articles:

				A. T. McLaren, P. A. Marsden, C. D. Mazer, A. J. Baker, D. J. Stewart, A. K. Y. Tsui, X. Li, Y. Yucel, M. Robb, S. R. Boyd, et al. Increased expression of HIF-1{alpha}, nNOS, and VEGF in the cerebral cortex of anemic rats Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R403 - R414. [Abstract] [Full Text] [PDF]

				C. W. Leffler, H. Parfenova, J. H. Jaggar, and R. Wang Carbon monoxide and hydrogen sulfide: gaseous messengers in cerebrovascular circulation J Appl Physiol, March 1, 2006; 100(3): 1065 - 1076. [Abstract] [Full Text] [PDF]

				H. Girouard and C. Iadecola Neurovascular coupling in the normal brain and in hypertension, stroke, and Alzheimer disease J Appl Physiol, January 1, 2006; 100(1): 328 - 335. [Abstract] [Full Text] [PDF]

				C. W. Leffler, L. Balabanova, A. L. Fedinec, and H. Parfenova Nitric oxide increases carbon monoxide production by piglet cerebral microvessels Am J Physiol Heart Circ Physiol, October 1, 2005; 289(4): H1442 - H1447. [Abstract] [Full Text] [PDF]

				L. Park, J. Anrather, P. Zhou, K. Frys, R. Pitstick, S. Younkin, G. A. Carlson, and C. Iadecola NADPH Oxidase-Derived Reactive Oxygen Species Mediate the Cerebrovascular Dysfunction Induced by the Amyloid {beta} Peptide J. Neurosci., February 16, 2005; 25(7): 1769 - 1777. [Abstract] [Full Text] [PDF]

				Y. Zhang, C. Forster, T. A. Milner, and C. Iadecola Attenuation of activity-induced increases in cerebellar blood flow by lesion of the inferior olive Am J Physiol Heart Circ Physiol, August 7, 2003; 285(3): H1177 - H1182. [Abstract] [Full Text] [PDF]