HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/1/H442    most recent 01071.2002v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (22) Citing Articles via Google Scholar Google Scholar Articles by Sheikh-Hamad, D. Articles by Youker, K. Search for Related Content PubMed PubMed Citation Articles by Sheikh-Hamad, D. Articles by Youker, K.

Stanniocalcin-1 is a naturally occurring L-channel inhibitor in cardiomyocytes: relevance to human heart failure

¹The Renal Section, ²Molecular Physiology and Biophysics, ³Cardiovascular Sciences Section, Baylor College of Medicine, ⁴Pathology and ⁵Cardiology Departments, University of Texas Health Sciences Center, Houston, Texas 77030; and ⁶The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark

Submitted 10 December 2002 ; accepted in final form 19 March 2003

Cardiomyocytes of the failing heart undergo profound phenotypic and structural changes that are accompanied by variations in the genetic program and profile of calcium homeostatic proteins. The underlying mechanisms for these changes remain unclear. Because the mammalian counterpart of the fish calcium-regulating hormone stanniocalcin-1 (STC1) is expressed in the heart, we reasoned that STC1 might play a role in the adaptive-maladaptive processes that lead to the heart failure phenotype. We examined the expression and localization of STC1 in cardiac tissue of patients with advanced heart failure before and after mechanical unloading using a left ventricular assist device (LVAD), and we compared the results with those of normal heart tissue. STC1 protein is markedly upregulated in cardiomyocytes and arterial walls of failing hearts pre-LVAD and is strikingly reduced after LVAD treatment. STC1 is diffusely expressed in cardiomyocytes, although nuclear predominance is apparent. Addition of recombinant STC1 to the medium of cultured rat cardiomyocytes slows their endogenous beating rate and diminishes the rise in intracellular calcium with each contraction. Furthermore, using whole cell patch-clamp studies in cultured rat cardiomyocytes, we find that addition of STC1 to the bath causes reversible inhibition of transmembrane calcium currents through L-channels. Our data suggest differential regulation of myocardial STC1 protein expression in heart failure. In addition, STC1 may regulate calcium currents in cardiomyocytes and may contribute to the alterations in calcium homeostasis of the failing heart.

calcium homeostasis; phenogenesis contribution; theraputic benifits

This article has been cited by other articles:

				D.-Y. Tseng, M.-Y. Chou, Y.-C. Tseng, C.-D. Hsiao, C.-J. Huang, T. Kaneko, and P.-P. Hwang Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2009; 296(3): R549 - R557. [Abstract] [Full Text] [PDF]

				C. Chen, M. S. Jamaluddin, S. Yan, D. Sheikh-Hamad, and Q. Yao Human Stanniocalcin-1 Blocks TNF-{alpha}-Induced Monolayer Permeability in Human Coronary Artery Endothelial Cells Arterioscler Thromb Vasc Biol, May 1, 2008; 28(5): 906 - 912. [Abstract] [Full Text] [PDF]

				J. A. Westberg, M. Serlachius, P. Lankila, and L. C. Andersson Hypoxic preconditioning induces elevated expression of stanniocalcin-1 in the heart Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1766 - H1771. [Abstract] [Full Text] [PDF]

				A. Chakraborty, H. Brooks, P. Zhang, W. Smith, M. R. McReynolds, J. B. Hoying, R. Bick, L. Truong, B. Poindexter, H. Lan, et al. Stanniocalcin-1 regulates endothelial gene expression and modulates transendothelial migration of leukocytes Am J Physiol Renal Physiol, February 1, 2007; 292(2): F895 - F904. [Abstract] [Full Text] [PDF]

				K. E. Wever, R. Masereeuw, D. S. Miller, X. M. Hang, and G. Flik Endothelin and calciotropic hormones share regulatory pathways in multidrug resistance protein 2-mediated transport Am J Physiol Renal Physiol, January 1, 2007; 292(1): F38 - F46. [Abstract] [Full Text] [PDF]

				A. D. Gagliardi, E. Y. W. Kuo, S. Raulic, G. F. Wagner, and G. E. DiMattia Human stanniocalcin-2 exhibits potent growth-suppressive properties in transgenic mice independently of growth hormone and IGFs Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E92 - E105. [Abstract] [Full Text] [PDF]

				D. Ito, J. R. Walker, C. S. Thompson, I. Moroz, W. Lin, M. L. Veselits, A. M. Hakim, A. A. Fienberg, and G. Thinakaran Characterization of Stanniocalcin 2, a Novel Target of the Mammalian Unfolded Protein Response with Cytoprotective Properties Mol. Cell. Biol., November 1, 2004; 24(21): 9456 - 9469. [Abstract] [Full Text] [PDF]

				J. Kanellis, R. Bick, G. Garcia, L. Truong, C. C. Tsao, D. Etemadmoghadam, B. Poindexter, L. Feng, R. J. Johnson, and D. Sheikh-Hamad Stanniocalcin-1, an inhibitor of macrophage chemotaxis and chemokinesis Am J Physiol Renal Physiol, February 1, 2004; 286(2): F356 - F362. [Abstract] [Full Text] [PDF]