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Attenuation of activity-induced increases in cerebellar blood flow by lesion of the inferior olive

Division of Neurobiology, Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, New York 10021

Submitted 18 March 2003 ; accepted in final form 29 April 2003

We sought to define the contribution of the climbing fibers (CF), one of the major inputs to Purkinje neurons, to the increase in cerebellar blood flow (BF_crb) produced by activation of the cerebellar cortex. The neurotoxin 3-acetylpyridine was used to lesion the inferior olive, the site from which the CF originate. Crus II, an area of the cerebellar cortex that receives sensory afferents from the perioral region, was activated by low-intensity stimulation of the upper lip (5–25 V and 4–16 Hz) in sham-lesioned and lesioned mice. BF_crb was recorded in crus II using a laser-Doppler flow probe. The increase in BF_crb produced by harmaline, an alkaloid that activates the CF, was abolished in lesioned mice (P > 0.05 vs. BF_crb before harmaline, n = 6), attesting to the effectiveness of the lesion. In sham-lesioned animals, upper lip stimulation increased BF_crb in crus II by 25 ± 2% (25 V and 10 Hz, n = 6). The rise in BF_crb was attenuated by 63 ± 7% (25 V and 10 Hz) in lesioned mice (P < 0.05, n = 6). In contrast, the increase in BF_crb produced by hypercapnia was not affected (P > 0.05). These data suggest that CF are responsible for a substantial portion of the increase in BF_crb produced by crus II activation. Thus the hemodynamic response evoked by functional activation of the cerebellar cortex reflects, in large part, CF activity.

cerebral circulation; 3-acetylpyridine; laser-Doppler flowmetry; functional hyperemia

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