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This Article Full Text Full Text (PDF) All Versions of this Article: 285/5/H1819    most recent 00383.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Schenkman, K. A. Articles by Feigl, E. O. Search for Related Content PubMed PubMed Citation Articles by Schenkman, K. A. Articles by Feigl, E. O.

Comparison of buffer and red blood cell perfusion of guinea pig heart oxygenation

Departments of ¹Pediatrics, ²Anesthesiology, ³Bioengineering, and ⁴Physiology and Biophysics, University of Washington, Seattle 98195; and ⁵Children's Hospital and Regional Medical Center, Seattle, Washington 98105

Submitted 24 April 2003 ; accepted in final form 14 July 2003

Myocardial mean myoglobin oxygen saturation was determined spectroscopically from isolated guinea pig hearts perfused with red blood cells during increasing hypoxia. These experiments were undertaken to compare intracellular myoglobin oxygen saturation in isolated hearts perfused with a modest concentration of red blood cells (5% hematocrit) with intracellular myoglobin saturation previously reported from traditional buffer-perfused hearts. Studies were performed at 37°C with hearts paced at 240 beats/min and a constant perfusion pressure of 80 cmH₂O. It was found that during perfusion with a hematocrit of 5%, baseline mean myoglobin saturation was 93% compared with 72% during buffer perfusion. Mean myoglobin saturation, ventricular function, and oxygen consumption remained fairly constant for arterial perfusate oxygen tensions above 100 mmHg and then decreased precipitously below 100 mmHg. In contrast, mean myoglobin saturation, ventricular function, and oxygen consumption began to decrease even at high oxygen tension with buffer perfusion. The present results demonstrate that perfusion with 5% red blood cells in the perfusate increases the baseline mean myoglobin saturation and better preserves cardiac function at low oxygen tension relative to buffer perfusion. These results suggest that caution should be used in extrapolating intracellular oxygen dynamics from buffer-perfused to blood-perfused hearts.

myocardial oxygen consumption; myoglobin oxygen saturation; optical spectroscopy; Langendorff-perfused heart

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