HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/6/H2327    most recent 00507.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Nozik-Grayck, E. Articles by Piantadosi, C. A. Search for Related Content PubMed PubMed Citation Articles by Nozik-Grayck, E. Articles by Piantadosi, C. A.

CALL FOR PAPERS

Bicarbonate-dependent superoxide release and pulmonary artery tone

Departments of Pediatrics and Medicine, Duke University Medical Center, Durham, North Carolina 27710

Submitted 2 June 2003 ; accepted in final form 2 July 2003

Pulmonary vasoconstriction is influenced by inactivation of nitric oxide (NO) with extracellular superoxide (). Because the short-lived anion cannot diffuse across plasma membranes, its release from vascular cells requires specialized mechanisms that have not been well delineated in the pulmonary circulation. We have shown that the bicarbonate anion exchange protein (AE2) expressed in the lung also exchanges for . Thus we determined whether release involved in pulmonary vascular tone depends on extracellular . We assessed endothelium-dependent vascular reactivity and release in the presence or absence of in pulmonary artery (PA) rings isolated from normal rats and those exposed to hypoxia for 3 days. Lack of extracellular in normal PA rings significantly attenuated endothelial release, opposed hypoxic vasoconstriction, and enhanced acetylcholine-mediated vasodilation. Release of was also inhibited by an AE2 inhibitor (SITS) and abolished in normoxia by an NO synthase inhibitor (N^G-nitro-L-arginine methyl ester). In contrast, hypoxia increased PA AE2 protein expression and release; the latter was not affected by N^G-nitro-L-arginine methyl ester or other inhibitors of enzymatic generation. Enhanced release by uncoupling NO synthase with geldanamycin was attenuated by hypoxia or by elimination. These results indicate that produced by endothelial NOS in normoxia and unidentified sources in hypoxia regulate pulmonary vascular tone via AE2.

anion exchange protein; nitric oxide; endothelial nitric oxide synthase; hypoxia

This article has been cited by other articles:

				F. Ketabchi, B. Egemnazarov, R. T. Schermuly, H. A. Ghofrani, W. Seeger, F. Grimminger, M. Shid-Moosavi, G. A. Dehghani, N. Weissmann, and N. Sommer Effects of hypercapnia with and without acidosis on hypoxic pulmonary vasoconstriction Am J Physiol Lung Cell Mol Physiol, November 1, 2009; 297(5): L977 - L983. [Abstract] [Full Text] [PDF]

				E. Nozik-Grayck, H. B. Suliman, S. Majka, J. Albietz, Z. Van Rheen, K. Roush, and K. R. Stenmark Lung EC-SOD overexpression attenuates hypoxic induction of Egr-1 and chronic hypoxic pulmonary vascular remodeling Am J Physiol Lung Cell Mol Physiol, September 1, 2008; 295(3): L422 - L430. [Abstract] [Full Text] [PDF]

				J. N. Edwards, W. A. Macdonald, C. van der Poel, and D. G. Stephenson O2bullet production at 37{degrees}C plays a critical role in depressing tetanic force of isolated rat and mouse skeletal muscle Am J Physiol Cell Physiol, August 1, 2007; 293(2): C650 - C660. [Abstract] [Full Text] [PDF]

				C. J. Taylor, P. A. Nicola, S. Wang, M. A. Barrand, and S. B. Hladky Transporters involved in regulation of intracellular pH in primary cultured rat brain endothelial cells J. Physiol., November 1, 2006; 576(3): 769 - 785. [Abstract] [Full Text] [PDF]

				J. Q. Liu, I. N. Zelko, E. M. Erbynn, J. S. K. Sham, and R. J. Folz Hypoxic pulmonary hypertension: role of superoxide and NADPH oxidase (gp91phox) Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L2 - L10. [Abstract] [Full Text] [PDF]

				J. W. Park, W.-N. Qi, Y. Cai, I. Zelko, J. Q. Liu, L.-E. Chen, J. R. Urbaniak, and R. J. Folz Skeletal muscle reperfusion injury is enhanced in extracellular superoxide dismutase knockout mouse Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H181 - H187. [Abstract] [Full Text] [PDF]