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This Article Full Text Full Text (PDF) All Versions of this Article: 285/6/H2543    most recent 00537.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Sakai, H. Articles by Tsuchida, E. Search for Related Content PubMed PubMed Citation Articles by Sakai, H. Articles by Tsuchida, E.

O₂ release from Hb vesicles evaluated using an artificial, narrow O₂-permeable tube: comparison with RBCs and acellular Hbs

¹Advanced Research Institute for Science and Engineering, Waseda University, Tokyo 169-8555; and ²Department of Physiology, School of Medicine, Ehime University, Shigenobu, Ehime 791-0295, Japan

Submitted 11 June 2003 ; accepted in final form 21 July 2003

A phospholipid vesicle that encapsulates a concentrated hemoglobin (Hb) solution and pyridoxal 5'-phosphate as an allosteric effector [Hb vesicle (HbV) diameter, 250 nm] has been developed to provide an O₂ carrying ability to plasma expanders. The O₂ release from flowing HbVs was examined using an O₂-permeable, fluorinated ethylenepropylene copolymer tube (inner diameter, 28 µm) exposed to a deoxygenated environment. Measurement of O₂ release was performed using an apparatus that consisted of an inverted microscope and a scanning-grating spectrophotometer with a photon-count detector, and the rate of O₂ release was determined based on the visible absorption spectrum in the Q band of Hb. HbVs and fresh human red blood cells (RBCs) were mixed in various volume ratios at a Hb concentration of 10 g/dl in isotonic saline that contained 5 g/dl albumin, and the suspension was perfused at the centerline flow velocity of 1 mm/s through the narrow tube. The mixtures of acellular Hb solution and RBCs were also tested. Because HbVs were homogeneously dispersed in the albumin solution, increasing the volume of the HbV suspension resulted in a thicker marginal RBC-free layer. Irrespective of the mixing ratio, the rate of O₂ release from the HbV/RBC mixtures was similar to that of RBCs alone. On the other hand, the addition of 50 vol% of acellular Hb solution to RBCs significantly enhanced the rate of deoxygenation. This outstanding difference in the rate of O₂ release between the HbV suspension and the acellular Hb solution should mainly be due to the difference in the particle size (250 vs. 7 nm) that affects their diffusion for the facilitated O₂ transport.

blood substitutes; red blood cells; hemoglobin; microcirculation; oxygenation; liposome

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