AJP - Heart AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 285: H2770-H2779, 2003. First published August 14, 2003; doi:10.1152/ajpheart.01114.2002
0363-6135/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/6/H2770    most recent
01114.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, Y. M.
Right arrow Articles by Dudley, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, Y. M.
Right arrow Articles by Dudley, S. C., Jr.

Characterization and regulation of T-type Ca2+ channels in embryonic stem cell-derived cardiomyocytes

Ying Ming Zhang,1,* Lijuan Shang,1,* Criss Hartzell,2 Michael Narlow,1 Leanne Cribbs,3 and Samuel C. Dudley, Jr.1

1Division of Cardiology, Atlanta Veterans Affairs Medical Center, Decatur 30033; 2Department of Cell Biology, Emory University, Atlanta, Georgia 30322; and 3Department of Medicine, Cardiovascular Institute, Loyola University Medical Center, Maywood, Illinois 60153

Submitted 27 December 2002 ; accepted in final form 6 August 2003

T-type Ca2+ channels may play a role in cardiac development. We studied the developmental regulation of the T-type currents (ICa,T) in cardiomyocytes (CMs) derived from mouse embryonic stem cells (ESCs). ICa,T was studied in isolated CMs by whole cell patch clamp. Subsequently, CMs were identified by the myosin light chain 2v-driven green fluorescent protein expression, and laser capture microdissection was used to isolate total RNA from groups of cells at various developmental time points. ICa,T showed characteristics of Cav3.1, such as resistance to Ni2+ block, and a transient increase during development, correlating with measures of spontaneous electrical activity. Real-time RT-PCR showed that Cav3.1 mRNA abundance correlated (r2 = 0.81) with ICa,T. The mRNA copy number was low at 7+4 days (2 copies/cell), increased significantly by 7+10 days (27/cell; P < 0.01), peaked at 7+16 days (174/cell), and declined significantly at 7+27 days (25/cell). These data suggest that ICa,T is developmentally regulated at the level of mRNA abundance and that this regulation parallels measures of pacemaker activity, suggesting that ICa,T might play a role in the spontaneous contractions during CM development.

growth and development; gene expression regulation; biological pacemaker; ion channel


Address for reprint requests and other correspondence: S. C. Dudley, Jr., Div. of Cardiology, Emory Univ./Atlanta VA Medical Center, 1670 Clairmont Rd., Rm. 111B, Decatur, GA 30033 (E-mail: sdudley{at}emory.edu).




This article has been cited by other articles:


Home page
 J. Physiol.Home page
Y. Qu, G. M. Whitaker, L. Hove-Madsen, G. F. Tibbits, and E. A. Accili
Hyperpolarization-activated cyclic nucleotide-modulated 'HCN' channels confer regular and faster rhythmicity to beating mouse embryonic stem cells
J. Physiol., February 1, 2008; 586(3): 701 - 716.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
L. L. Shang, A. E. Pfahnl, S. Sanyal, Z. Jiao, J. Allen, K. Banach, J. Fahrenbach, D. Weiss, W. R. Taylor, A. M. Zafari, et al.
Human Heart Failure Is Associated With Abnormal C-Terminal Splicing Variants in the Cardiac Sodium Channel
Circ. Res., November 26, 2007; 101(11): 1146 - 1154.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
N. Kapur and K. Banach
Inositol-1,4,5-trisphosphate-mediated spontaneous activity in mouse embryonic stem cell-derived cardiomyocytes
J. Physiol., June 15, 2007; 581(3): 1113 - 1127.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Kapur, G. A. Mignery, and K. Banach
Cell cycle-dependent calcium oscillations in mouse embryonic stem cells
Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1510 - C1518.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
X. Chen, R. M. Wilson, H. Kubo, R. M. Berretta, D. M. Harris, X. Zhang, N. Jaleel, S. M. MacDonnell, C. Bearzi, J. Tillmanns, et al.
Adolescent Feline Heart Contains a Population of Small, Proliferative Ventricular Myocytes With Immature Physiological Properties
Circ. Res., March 2, 2007; 100(4): 536 - 544.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. M. White and W. C. Claycomb
Embryonic stem cells form an organized, functional cardiac conduction system in vitro
Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H670 - H679.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.