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Relationship between erythrocyte aggregate size and flow rate in skeletal muscle venules

¹Department of Bioengineering, University of California, San Diego, La Jolla, California 92093; and ²Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205

Submitted 24 June 2003 ; accepted in final form 3 September 2003

In previous studies we showed that intravenous infusion of Dextran 500 in the rat causes blunting of the velocity profile of red blood cells in venules at low shear rates. To determine whether this blunting is associated with the formation of red blood cell aggregates, we measured the length and width of particles in the venular flow stream at systemic hematocrits up to 20% with a high-speed video camera and a new image analysis technique. Data were obtained at various shear rates under normal (nonaggregating) conditions as well as after infusion of Dextran 500. Under normal conditions, particle length (parallel to the vessel axis) was 6.5 ± 2.7 µm and width (perpendicular to the axis) was 6.1 ± 1.7 µm, in agreement with published dimensions of individual red blood cells for this species. After Dextran 500 infusion, particle length and width increased significantly to 8.7 ± 5.1 and 10.4 ± 4.4 µm, respectively. Particle dimensions were greater in the central region of the flow stream for both normal and dextran-treated blood and increased at low flow rates with dextran-treated blood. This study provides direct confirmation of aggregate formation at low shear in venules with high-molecular-weight dextran as well as an estimate of aggregate size and range.

venous vascular resistance; in vivo blood rheology; in vivo microscopy; red blood cell aggregation

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