AJP - Heart AJP citation statistics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 286: H359-H367, 2004. First published September 11, 2003; doi:10.1152/ajpheart.00491.2003
0363-6135/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
286/1/H359    most recent
00491.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (29)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, X.
Right arrow Articles by Goligorsky, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, X.
Right arrow Articles by Goligorsky, M. S.

Atomic force microscopy measurement of leukocyte-endothelial interaction

Xiaohui Zhang,1 Aileen Chen,1 Dina De Leon,2 Hong Li,4 Eisei Noiri,2 Vincent T. Moy,1 and Michael S. Goligorsky4

1Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, Florida 33101; 2Department of Medicine, The University of Tokyo, Tokyo 113-8655, Japan; 3Department of Pediatrics, State University of New York, Stony Brook 11794; and 4Departments of Medicine and Pharmacology, New York Medical College, Valhalla, New York 10595

Submitted 28 May 2003 ; accepted in final form 10 September 2003

Leukocyte adhesion to vascular endothelium is a key initiating step in the pathogenesis of many inflammatory diseases. In this study, we present real-time force measurements of the interaction between monocytic human promyelocytic leukemia cells (HL-60) cells and a monolayer of human umbilical vein endothelial cells (HUVECs) by using atomic force microscopy (AFM). The detachment of HL-60-HUVEC conjugates involved a series of rupture events with force transitions of 40–100 pN. The integrated force of these rupture events provided a quantitative measure of the adhesion strength on a whole cell level. The AFM measurements revealed that HL-60 adhesion is heightened in the borders formed by adjacent HUVECs. The average force and mechanical work required to detach a single HL-60 from the borders of a tumor necrosis factor-{alpha}-activated HUVEC layer were twice as high as those of the HUVEC bodies. HL-60 adhesion to the monolayer was significantly reduced by a monoclonal antibody against {beta}1-integrins and partially inhibited by antibodies against selectins ICAM-1 and VCAM-1 but was not affected by anti-{alpha}V{beta}3. Interestingly, adhesion was also inhibited in a dose-dependent manner (IC50 {approx} 100 nM) by a cyclic arginine-glycine-aspartic acid (cRGD) peptide. This effect was mediated via interfering with the VLA-4-VCAM-1 binding. In parallel measurements, transmigration of HL-60 cells across a confluent HUVEC monolayer was inhibited by the cRGD peptide and by both anti-{beta}1 and anti-{alpha}V{beta}3 antibodies. In conclusion, these data demonstrate the role played by {beta}1-integrins in leukocyte-endothelial adhesion and transmigration and the role played by {alpha}V{beta}3 in transmigration, thus underscoring the high efficacy of cRGD peptide in blocking both the adhesion and transmigration of monocytes.

integrins; cell-cell adhesion; arginine-glycine-aspartic acid peptide


Address for reprint requests and other correspondence: V. T. Moy, Dept. of Physiology and Biophysics, Univ. of Miami School of Medicine, Miami, FL 33101-6430 (E-mail: vmoy{at}miami.edu) or M. S. Goligorsky, Dept. of Medicine, New York Medical College, Valhalla, NY 10595 (E-mail: Michael_Goligorsky{at}nymc.edu).




This article has been cited by other articles:


Home page
 Biophys. JHome page
A. Rabodzey, P. Alcaide, F. W. Luscinskas, and B. Ladoux
Mechanical Forces Induced by the Transendothelial Migration of Human Neutrophils
Biophys. J., August 1, 2008; 95(3): 1428 - 1438.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. Schmitz, M. Benoit, and K.-E. Gottschalk
The Viscoelasticity of Membrane Tethers and Its Importance for Cell Adhesion
Biophys. J., August 1, 2008; 95(3): 1448 - 1459.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A. P. Gunning, S. Chambers, C. Pin, A. L. Man, V. J. Morris, and C. Nicoletti
Mapping specific adhesive interactions on living human intestinal epithelial cells with atomic force microscopy
FASEB J, July 1, 2008; 22(7): 2331 - 2339.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
J. H. Rhim and G. Tosato
Targeting the Tumor Vasculature to Improve the Efficacy of Oncolytic Virus Therapy
J Natl Cancer Inst, December 5, 2007; 99(23): 1739 - 1741.
[Full Text] [PDF]

Home page
 Biophys. JHome page
P. Roca-Cusachs, I. Almendros, R. Sunyer, N. Gavara, R. Farre, and D. Navajas
Rheology of Passive and Adhesion-Activated Neutrophils Probed by Atomic Force Microscopy
Biophys. J., November 1, 2006; 91(9): 3508 - 3518.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Subramanian, R. Parthasarathy, S. Sen, E. T. Boder, and D. E. Discher
Species- and cell type-specific interactions between CD47 and human SIRP{alpha}
Blood, March 15, 2006; 107(6): 2548 - 2556.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Elitok, S. V. Brodsky, D. Patschan, T. Orlova, K. M. Lerea, P. Chander, and M. S. Goligorsky
Cyclic arginine-glycine-aspartic acid peptide inhibits macrophage infiltration of the kidney and carotid artery lesions in apo-E-deficient mice
Am J Physiol Renal Physiol, January 1, 2006; 290(1): F159 - F166.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
R. I. Litvinov, J. S. Bennett, J. W. Weisel, and H. Shuman
Multi-Step Fibrinogen Binding to the Integrin {alpha}IIb{beta}3 Detected Using Force Spectroscopy
Biophys. J., October 1, 2005; 89(4): 2824 - 2834.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
M. S. Goligorsky
Whispers and shouts in the pathogenesis of acute renal ischaemia
Nephrol. Dial. Transplant., February 1, 2005; 20(2): 261 - 266.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.