AJP - Heart AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 286: H1107-H1113, 2004. First published November 26, 2003; doi:10.1152/ajpheart.00656.2003
0363-6135/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
286/3/H1107    most recent
00656.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dubrovska, G.
Right arrow Articles by Gollasch, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dubrovska, G.
Right arrow Articles by Gollasch, M.

Mechanisms of ADRF release from rat aortic adventitial adipose tissue

Galyna Dubrovska, Stefan Verlohren, Friedrich C. Luft, and Maik Gollasch

Franz Volhard Clinic and Max Delbrück Center for Molecular Medicine, Charité University Hospitals, Humboldt University of Berlin, HELIOS Klinikum Berlin, 13125 Berlin, Germany

Submitted 14 July 2003 ; accepted in final form 17 November 2003

Blood vessels are surrounded by variable amounts of adipose tissue. We showed earlier that adventitial adipose tissue inhibits rat aortic contraction by release of a transferable factor, adventitium-derived relaxing factor (ADRF), which activates smooth muscle K+ channels. However, little is known about the mechanisms of ADRF release. Using isolated rat aortic rings and isometric contraction measurements, we show that ADRF release depends on extracellular [Ca2+] (EC50 ~ 4.7 mM). ADRF effects do not involve neuronal presynaptic N-type Ca2+ and Na+ channels or vanilloid, cannabinoid, and CGRP receptors. ADRF release is strongly inhibited by the protein tyrosine kinase inhibitors genistein and tyrphostin A25. In contrast, daidzein, an inactive genistein analog, and the protein tyrosine kinase inhibitor ST638 had no effect. Protein kinase A inhibition by H89 also inhibited ADRF release, whereas the protein kinase G inhibitor KT-5823 had no effect. We propose that ADRF release is Ca2+ dependent and is regulated by intracellular signaling pathways involving tyrosine kinase and protein kinase A. Furthermore, ADRF release does not depend on perivascular nerve endings.

adipocyte-derived relaxing factor; smooth muscle; tyrosine kinase; protein kinase A; obesity; vascular dysfunction; arterial tone; adventitia-derived relaxing factor


Address for reprint requests and other correspondence: M. Gollasch, Franz Volhard Clinic, Wiltbergstrasse 50, 13125 Berlin, Germany (E-mail: gollasch{at}fvk-berlin.de).




This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. A. Payne, H. G. Bohlen, U. D. Dincer, L. Borbouse, and J. D. Tune
Periadventitial adipose tissue impairs coronary endothelial function via PKC-{beta}-dependent phosphorylation of nitric oxide synthase
Am J Physiol Heart Circ Physiol, July 1, 2009; 297(1): H460 - H465.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
A. S. Greenstein, K. Khavandi, S. B. Withers, K. Sonoyama, O. Clancy, M. Jeziorska, I. Laing, A. P. Yates, P. W. Pemberton, R. A. Malik, et al.
Local Inflammation and Hypoxia Abolish the Protective Anticontractile Properties of Perivascular Fat in Obese Patients
Circulation, March 31, 2009; 119(12): 1661 - 1670.
[Abstract] [Full Text] [PDF]

Home page
 Eur. J. Cardiothorac. Surg.Home page
M. Malinowski, M. A. Deja, K. S. Golba, T. Roleder, J. Biernat, and S. Wos
Perivascular tissue of internal thoracic artery releases potent nitric oxide and prostacyclin-independent anticontractile factor
Eur. J. Cardiothorac. Surg., February 1, 2008; 33(2): 225 - 231.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
F. A. Auger, P. D'Orleans-Juste, and L. Germain
Adventitia contribution to vascular contraction: Hints provided by tissue-engineered substitutes
Cardiovasc Res, September 1, 2007; 75(4): 669 - 678.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
G. Fesus, G. Dubrovska, K. Gorzelniak, R. Kluge, Y. Huang, F. C. Luft, and M. Gollasch
Adiponectin is a novel humoral vasodilator
Cardiovasc Res, September 1, 2007; 75(4): 719 - 727.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
Y.-J. Gao, K. Takemori, L.-Y. Su, W.-S. An, C. Lu, A. M. Sharma, and R. M.K.W. Lee
Perivascular adipose tissue promotes vasoconstriction: the role of superoxide anion
Cardiovasc Res, July 15, 2006; 71(2): 363 - 373.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
Y.-J. Gao, Z.-h. Zeng, K. Teoh, A. M. Sharma, L. Abouzahr, I. Cybulsky, A. Lamy, L. Semelhago, and R. M.K.W. Lee
Perivascular adipose tissue modulates vascular function in the human internal thoracic artery
J. Thorac. Cardiovasc. Surg., October 1, 2005; 130(4): 1130 - 1136.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. M. Gauthier, D. V. Baewer, S. Hittner, C. J. Hillard, K. Nithipatikom, D. S. Reddy, J. R. Falck, and W. B. Campbell
Endothelium-derived 2-arachidonylglycerol: an intermediate in vasodilatory eicosanoid release in bovine coronary arteries
Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1344 - H1351.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.