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Microlymphatic and tissue oxygen tension in the rat mesentery

¹Department of Bioengineering, University of California-San Diego, La Jolla, California 92093; and ²Department of Mechanical Engineering, Universidad de los Andes, Bogotá, Colombia

Submitted 25 September 2003 ; accepted in final form 12 November 2003

Oxygen phosphorescence quenching was used to measure tissue PO₂ of lymphatic vessels of 43.6 ± 23.1 µm (mean ± SD) diameter in tissue locations of the rat mesentery classified according to anatomic location. Lymph and adipose tissue PO₂ were 20.6 ± 9.1 and 34.1 ± 7.8 mmHg, respectively, with the difference being statistically significant. Rare microlymphatic vessels in connective tissue not surrounded by microvessels had a PO₂ of 0.8 ± 0.2 mmHg, whereas the surrounding tissue PO₂ was 3.0 ± 3.2 mmHg, with both values being significantly lower than those of adipose tissue. Lower of lymph fluid PO₂ relative to the surrounding tissue was also evident in paired measurements of PO₂ in the lymphatic vessels and perilymphatic adipose tissue, which was significantly lower than the PO₂ in paired adipose tissue. The PO₂ of the lymphatic fluid of the mesenteric microlymphatics is consistently lower than that of the surrounding adipose tissue by 11 mmHg; therefore, lymph fluid has the lowest PO₂ of this tissue. The disparity between lymph and tissue PO₂ is attributed to the microlymphatic vessel wall and lymphocyte oxygen consumption.

lymph oxygen; lymphatic wall; oxygen consumption

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