| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Department of Molecular and Integrative Physiology and 2Department of Surgery (Vascular), University of Michigan Medical School, Ann Arbor 48109; 3Department of Surgery, William Beaumont Hospital, Royal Oak 48073; 4Veterans Administration, Ann Arbor Healthcare System, Ann Arbor, Michigan 48105
Submitted 18 November 2003 ; accepted in final form 9 February 2004
Radiotelemetry of mouse blood pressure accurately monitors systolic pressure, diastolic pressure, heart rate, and locomotor activity but requires surgical implantation. Noninvasive measurements of indirect systolic blood pressure have long been available for larger rodents and now are being reported more frequently for mice. This study compared mouse systolic arterial blood pressure measurements using implanted radiotelemetry pressure transducer with simultaneous tail-cuff measurements in the same unanesthetized mice. The pressure range for comparison was extended by inducing experimental hypertension or by observations of circadian elevations between 3 AM and 6 AM. Both trained and untrained tail-cuff operators used both instruments. Every effort was made to follow recommended manufacturer's instructions. With the initial flow-based tail-cuff instrument, we made 671 comparisons (89 sessions) and found the slope of the linear regression to be 0.118, suggesting poor agreement. In an independent assessment, 277 comparisons (35 sessions) of radiotelemetry measurements with the pulse based tail-cuff instrument were made. The slope of the linear regression of the simultaneous measurements of systolic pressures was 0.98, suggesting agreement. Bland-Altman analysis also supported our interpretation of the linear regression. Thus although reliable systolic pressure measurements are possible with either tail-cuff or radiotelemetry techniques, in our hands some tail-cuff instruments fail to accurately detect elevated blood pressures. These data, however, do not distinguish whether this instrument-specific tail-cuff failure was due to operator or instrument inadequacies. We strongly advise investigators to obtain an independent and simultaneous validation of tail-cuff determinations of mouse blood pressure before making critical genotyping determinations.
rodent blood pressure; indirect and direct blood pressure measurement technique
This article has been cited by other articles:
|
|
 |
|
  L.-F. Zhang, J.-H. Cheng, X. Liu, S. Wang, Y. Liu, H.-B. Lu, and J. Ma Cardiovascular changes of conscious rats after simulated microgravity with and without daily -Gx gravitation J Appl Physiol, October 1, 2008; 105(4): 1134 - 1145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. Shi, X. R. Cao, E. M. Sweezer, T. S. Kinney, N. R. Williams, R. F. Husted, R. Nair, R. M. Weiss, R. A. Williamson, C. D. Sigmund, et al. Salt-sensitive hypertension and cardiac hypertrophy in mice deficient in the ubiquitin ligase Nedd4-2 Am J Physiol Renal Physiol, August 1, 2008; 295(2): F462 - F470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sachidanandam, M. M. Elgebaly, A. K. Harris, J. R. Hutchinson, E. M. Mezzetti, V. Portik-Dobos, and A. Ergul Effect of chronic and selective endothelin receptor antagonism on microvascular function in Type 2 diabetes Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2743 - H2749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Lloyd, J. McCormick, J. Helmering, K. W. Kim, M. Wang, P. Fordstrom, S. A. Kaufman, R. A. Lindberg, and M. M. Veniant Generation and characterization of two novel mouse models exhibiting the phenotypes of the metabolic syndrome: Apob48-/-Lepob/ob mice devoid of ApoE or Ldlr Am J Physiol Endocrinol Metab, March 1, 2008; 294(3): E496 - E505. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Rigsby, A. E. Burch, S. Ogbi, D. M. Pollock, and A. M. Dorrance Intact female stroke-prone hypertensive rats lack responsiveness to mineralocorticoid receptor antagonists Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1754 - R1763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Baserga, M. A. Hale, Z. M. Wang, X. Yu, C. W. Callaway, R. A. McKnight, and R. H. Lane Uteroplacental insufficiency alters nephrogenesis and downregulates cyclooxygenase-2 expression in a model of IUGR with adult-onset hypertension Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R1943 - R1955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sjoblom and O. Nylander Isoflurane-induced acidosis depresses basal and PGE2-stimulated duodenal bicarbonate secretion in mice Am J Physiol Gastrointest Liver Physiol, March 1, 2007; 292(3): G899 - G904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. F. Bodary, Y. Shen, M. Ohman, K. L. Bahrou, F. B. Vargas, S. S. Cudney, K. J. Wickenheiser, M. G. Myers Jr, and D. T. Eitzman Leptin Regulates Neointima Formation After Arterial Injury Through Mechanisms Independent of Blood Pressure and the Leptin Receptor/STAT3 Signaling Pathways Involved in Energy Balance Arterioscler. Thromb. Vasc. Biol., January 1, 2007; 27(1): 70 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Ovechkin, N. Tyagi, U. Sen, D. Lominadze, M. M. Steed, K. S. Moshal, and S. C. Tyagi 3-Deazaadenosine mitigates arterial remodeling and hypertension in hyperhomocysteinemic mice Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L905 - L911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, P. J. Flannery, K. Athirakul, S. R. Dunn, W. M. Kourany, and R. F. Spurney G{alpha}q-dependent signaling cascades stimulate water-seeking behavior Am J Physiol Renal Physiol, October 1, 2006; 291(4): F781 - F789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Elrod, J. J.M. Greer, N. S. Bryan, W. Langston, J. F. Szot, H. Gebregzlabher, S. Janssens, M. Feelisch, and D. J. Lefer Cardiomyocyte-Specific Overexpression of NO Synthase-3 Protects Against Myocardial Ischemia-Reperfusion Injury Arterioscler. Thromb. Vasc. Biol., July 1, 2006; 26(7): 1517 - 1523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ohashi, S. Kihara, N. Ouchi, M. Kumada, K. Fujita, A. Hiuge, T. Hibuse, M. Ryo, H. Nishizawa, N. Maeda, et al. Adiponectin Replenishment Ameliorates Obesity-Related Hypertension Hypertension, June 1, 2006; 47(6): 1108 - 1116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Wolf 'As time goes by': angiotensin II-mediated transactivation of the EGF receptor comes of age Nephrol. Dial. Transplant., October 1, 2005; 20(10): 2050 - 2053. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Iida, G. L. Baumbach, J. L. Lavoie, F. M. Faraci, C. D. Sigmund, and D. D. Heistad Spontaneous Stroke in a Genetic Model of Hypertension in Mice Stroke, June 1, 2005; 36(6): 1253 - 1258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. F. Benjamim, C. Canetti, F. Q. Cunha, S. L. Kunkel, and M. Peters-Golden Opposing and Hierarchical Roles of Leukotrienes in Local Innate Immune versus Vascular Responses in a Model of Sepsis J. Immunol., February 1, 2005; 174(3): 1616 - 1620. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
