AJP - Heart Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 287: H187-H195, 2004; doi:10.1152/ajpheart.01058.2003
0363-6135/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (24)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, J.-X.
Right arrow Articles by Lin, P. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, J.-X.
Right arrow Articles by Lin, P. C.

Dual functional roles of Tie-2/angiopoietin in TNF-{alpha}-mediated angiogenesis

Jian-Xiong Chen,1 Ying Chen,2 Laura DeBusk,3 Wenyu Lin,1 and Pengnain Charles Lin1,2,3

Departments of 1Radiation Oncology, 2Cell Biology, and 3Cancer Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232

Submitted 24 November 2003 ; accepted in final form 19 February 2004

Inflammation and angiogenesis are associated with pathological disorders. TNF-{alpha} is a major inflammatory cytokine that also regulates angiogenesis. TNF-{alpha} has been shown to regulate Tie-2 and angiopoietin (Ang) expression, but the functional significance is less clear. In this study, we showed that TNF-{alpha} induced a weak angiogenic response in a mouse cornea assay. Systemic overexpression of Ang-1 or Ang-2 dramatically increased corneal angiogenesis induced by TNF-{alpha}. In the absence of TNF-{alpha}, neither Ang-1 nor Ang-2 promoted corneal angiogenesis. Low doses (0–25 ng/ml) of TNF-{alpha} increased vascular branch formation of cultured endothelial cells. Overexpression of Ang-1 or Ang-2 enhanced the effects of TNF-{alpha}. These data suggest that Tie-2 signaling synergistically amplifies and participates in TNF-{alpha}-mediated angiogenesis. In addition, high doses (≥50 ng/ml) of TNF-{alpha} induced apoptosis in endothelial cells, but addition of Ang-1 or Ang-2 significantly reduced cell death. Enhanced endothelial cell survival was correlated with Akt phosphorylation. Collectively, our data reveal dual functional roles of Tie-2: low doses enhance TNF-{alpha}-induced angiogenesis, and high doses attenuate TNF-{alpha}-induced cell death. The study provides evidence supporting a role for Tie-2 in inflammatory angiogenesis.

inflammation; cell death


Address for reprint requests and other correspondence: P. C. Lin, Vanderbilt Univ. Medical Center, 2220 Pierce Ave., Preston Research Bldg., Rm. 315, Nashville, TN 37232 (E-mail: charles.lin{at}vanderbilt.edu).




This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Q.-h. Tuo, H. Zeng, A. Stinnett, H. Yu, J. L. Aschner, D.-F. Liao, and J.-X. Chen
Critical role of angiopoietins/Tie-2 in hyperglycemic exacerbation of myocardial infarction and impaired angiogenesis
Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2547 - H2557.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S. Shilo, S. Roy, S. Khanna, and C. K. Sen
Evidence for the Involvement of miRNA in Redox Regulated Angiogenic Response of Human Microvascular Endothelial Cells
Arterioscler. Thromb. Vasc. Biol., March 1, 2008; 28(3): 471 - 477.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
A. C. Aplin, M. Gelati, E. Fogel, E. Carnevale, and R. F. Nicosia
Angiopoietin-1 and vascular endothelial growth factor induce expression of inflammatory cytokines before angiogenesis
Physiol Genomics, October 3, 2006; 27(1): 20 - 28.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
G. M. Borthwick, A. S. Johnson, M. Partington, J. Burn, R. Wilson, and H. M. Arthur
Therapeutic levels of aspirin and salicylate directly inhibit a model of angiogenesis through a Cox-independent mechanism
FASEB J, October 1, 2006; 20(12): 2009 - 2016.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Harfouche and S. N. A. Hussain
Signaling and regulation of endothelial cell survival by angiopoietin-2
Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1635 - H1645.
[Abstract] [Full Text] [PDF]

Home page
 JNMHome page
P. Kunz, J. Hoffend, A. Altmann, A. Dimitrakopoulou-Strauss, D. Koczan, M. Eisenhut, G. A. Bonaterra, T. J. Dengler, W. Mier, U. Haberkorn, et al.
Angiopoietin-2 Overexpression in Morris Hepatoma Results in Increased Tumor Perfusion and Induction of Critical Angiogenesis-Promoting Genes
J. Nucl. Med., September 1, 2006; 47(9): 1515 - 1524.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. I. Nykanen, K. Pajusola, R. Krebs, M. A.I. Keranen, O. Raisky, P. K. Koskinen, K. Alitalo, and K. B. Lemstrom
Common Protective and Diverse Smooth Muscle Cell Effects of AAV-Mediated Angiopoietin-1 and -2 Expression in Rat Cardiac Allograft Vasculopathy
Circ. Res., June 9, 2006; 98(11): 1373 - 1380.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
N. P.J. Brindle, P. Saharinen, and K. Alitalo
Signaling and Functions of Angiopoietin-1 in Vascular Protection
Circ. Res., April 28, 2006; 98(8): 1014 - 1023.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. Frantz, K. A. Vincent, O. Feron, and R. A. Kelly
Innate Immunity and Angiogenesis
Circ. Res., January 7, 2005; 96(1): 15 - 26.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.