HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) A corrigendum has been published All Versions of this Article: 287/1/H203    most recent 01237.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (31) Citing Articles via Google Scholar Google Scholar Articles by Titze, J. Articles by Hilgers, K. F. Search for Related Content PubMed PubMed Citation Articles by Titze, J. Articles by Hilgers, K. F.

Glycosaminoglycan polymerization may enable osmotically inactive Na⁺ storage in the skin

¹Department of Medicine IV, Friedrich-Alexander-University Erlangen-Nürnberg, D-90471 Nürnberg; Departments of ²Anatomy and ⁴Biochemistry, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, D-14195 Berlin; and ³Department of Chemistry and Physics, Federal Center for Meat Research, D-95326 Kulmbach, Germany

Submitted 29 December 2003 ; accepted in final form 13 February 2004

Osmotically inactive skin Na⁺ storage is characterized by Na⁺ accumulation without water accumulation in the skin. Negatively charged glycosaminoglycans (GAGs) may be important in skin Na⁺ storage. We investigated changes in skin GAG content and key enzymes of GAG chain polymerization during osmotically inactive skin Na⁺ storage. Female Sprague-Dawley rats were fed a 0.1% or 8% NaCl diet for 8 wk. Skin GAG content was measured by Western blot analysis. mRNA content of key dermatan sulfate polymerization enzymes was measured by real-time PCR. The Na⁺ concentration in skin was determined by dry ashing. Skin Na⁺ concentration during osmotically inactive Na⁺ storage was 180–190 mmol/l. Increasing skin Na⁺ coincided with increasing GAG content in cartilage and skin. Dietary NaCl loading coincided with increased chondroitin synthase mRNA content in the skin, whereas xylosyl transferase, biglycan, and decorin content were unchanged. We conclude that osmotically inactive skin Na⁺ storage is an active process characterized by an increased GAG content in the reservoir tissue. Inhibition or disinhibition of GAG chain polymerization may regulate osmotically inactive Na⁺ storage.

hypertension; extracellular matrix; chondroitin synthase; elongation enzymes

This article has been cited by other articles:

				P. Gross, T. Marczewski, and K. Herbrig The vaptans ante portas: a status report Nephrol. Dial. Transplant., May 1, 2009; 24(5): 1371 - 1373. [Full Text] [PDF]

				A. Ziomber, A. Machnik, A. Dahlmann, P. Dietsch, F.-X. Beck, H. Wagner, K. F. Hilgers, F. C. Luft, K.-U. Eckardt, and J. Titze Sodium-, potassium-, chloride-, and bicarbonate-related effects on blood pressure and electrolyte homeostasis in deoxycorticosterone acetate-treated rats Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1752 - F1763. [Abstract] [Full Text] [PDF]

				S. Shaldon and J. Vienken The long forgotten salt factor and the benefits of using a 5-g-salt-restricted diet in all ESRD patients Nephrol. Dial. Transplant., July 1, 2008; 23(7): 2118 - 2120. [Full Text] [PDF]

				M. Schafflhuber, N. Volpi, A. Dahlmann, K. F. Hilgers, F. Maccari, P. Dietsch, H. Wagner, F. C. Luft, K.-U. Eckardt, and J. Titze Mobilization of osmotically inactive Na+ by growth and by dietary salt restriction in rats Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1490 - F1500. [Abstract] [Full Text] [PDF]

				M. H. Rosner and J. Kirven Exercise-Associated Hyponatremia Clin. J. Am. Soc. Nephrol., January 1, 2007; 2(1): 151 - 161. [Abstract] [Full Text] [PDF]

				E. Seeliger, M. Ladwig, and H. W. Reinhardt Are large amounts of sodium stored in an osmotically inactive form during sodium retention? Balance studies in freely moving dogs Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1429 - R1435. [Abstract] [Full Text] [PDF]

				M. K. Nguyen and I. Kurtz Quantitative interrelationship between Gibbs-Donnan equilibrium, osmolality of body fluid compartments, and plasma water sodium concentration J Appl Physiol, April 1, 2006; 100(4): 1293 - 1300. [Abstract] [Full Text] [PDF]

				K. D.C., L. D.F., W. J., H. D., Y. X, T. J., B. K., S. M., D. P., L. R., et al. Accessory Renal Arteries--Mostly, But Not Always, Innocuous: Renin-Dependent Hypertension Caused by Nonfocal Stenotic Aberrant Renal Arteries--Proof of a New Syndrome. Hypertension 46: 380-385, 2005 J. Am. Soc. Nephrol., January 1, 2006; 17(1): 3 - 11. [Full Text] [PDF]

				J. Titze, K. Bauer, M. Schafflhuber, P. Dietsch, R. Lang, K. H. Schwind, F. C. Luft, K.-U. Eckardt, and K. F. Hilgers Internal sodium balance in DOCA-salt rats: a body composition study Am J Physiol Renal Physiol, October 1, 2005; 289(4): F793 - F802. [Abstract] [Full Text] [PDF]

				G. F. Mitchell, A. L. DeStefano, M. G. Larson, E. J. Benjamin, M.-H. Chen, R. S. Vasan, J. A. Vita, and D. Levy Heritability and a Genome-Wide Linkage Scan for Arterial Stiffness, Wave Reflection, and Mean Arterial Pressure: The Framingham Heart Study Circulation, July 12, 2005; 112(2): 194 - 199. [Abstract] [Full Text] [PDF]