HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 287/1/H419    most recent 00699.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Bäck, M. Articles by Sakata, K. Search for Related Content PubMed PubMed Citation Articles by Bäck, M. Articles by Sakata, K.

Leukotriene B₄ is an indirectly acting vasoconstrictor in guinea pig aorta via an inducible type of BLT receptor

¹Division of Physiology, The National Institute of Environmental Medicine, Karolinska Institutet; and ²Division of Physiological Chemistry 2, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden

Submitted 23 July 2003 ; accepted in final form 4 March 2004

Leukotriene B₄ (LTB₄) is a potent leukocyte chemoattractant recently implicated in the pathogenesis of atherosclerosis. The aim of this study was to assess the effects of LTB₄ on isolated aortic preparations. Rings of guinea pig aorta were challenged with LTB₄ for recording mechanical responses and measurements of mediator release, and LTB₄ receptor (BLT₁) expression was assessed by RT-PCR. Single concentrations of LTB₄ induced concentration-dependent contractions that were inhibited by treatment with antihistamines, indomethacin, or the thromboxane receptor antagonist BAYu3405 as well as by denudation of endothelium. In addition, LTB₄ increased the release of histamine and thromboxane in the bath. The contractions induced by LTB₄ were inhibited by either the unselective BLT receptor antagonist ONO-4057 or the selective BLT₁ receptor antagonist U-75302. Pretreatment with all-trans-retinoic acid enhanced the contractions and the release of histamine induced by LTB₄, without affecting either the contractions induced by histamine or the histamine release evoked by calcium ionophore A23187. Analysis by RT-PCR indicated the expression of a BLT₁ receptor in the guinea pig aorta and that BLT₁ receptor mRNA was upregulated after treatment with retinoic acid. These results suggest that LTB₄ contracts the guinea pig aorta via an indirect mechanism involving the release of histamine and thromboxane and that this BLT₁ receptor-mediated response can be upregulated by all-trans-retinoic acid.

histamine; retinoic acid; endothelium; thromboxane

This article has been cited by other articles:

				E. Sanchez-Galan, A. Gomez-Hernandez, C. Vidal, J. L. Martin-Ventura, L. M. Blanco-Colio, B. Munoz-Garcia, L. Ortega, J. Egido, and J. Tunon Leukotriene B4 enhances the activity of nuclear factor-{kappa}B pathway through BLT1 and BLT2 receptors in atherosclerosis Cardiovasc Res, January 1, 2009; 81(1): 216 - 225. [Abstract] [Full Text] [PDF]