| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
CALL FOR PAPERS
Cellular Plasticity in the Cardiovascular System
1Vascular Biology Program, Departments of 2Surgery and 3Cardiac Surgery, Children's Hospital, and 4Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115
Submitted 29 December 2003 ; accepted in final form 22 March 2004
Tissue engineering may offer patients new options when replacement or repair of an organ is needed. However, most tissues will require a microvascular network to supply oxygen and nutrients. One strategy for creating a microvascular network would be promotion of vasculogenesis in situ by seeding vascular progenitor cells within the biopolymeric construct. To pursue this strategy, we isolated CD34+/CD133+ endothelial progenitor cells (EPC) from human umbilical cord blood and expanded the cells ex vivo as EPC-derived endothelial cells (EC). The EPC lost expression of the stem cell marker CD133 but continued to express the endothelial markers KDR/VEGF-R2, VE-cadherin, CD31, von Willebrand factor, and E-selectin. The cells were also shown to mediate calcium-dependent adhesion of HL-60 cells, a human promyelocytic leukemia cell line, providing evidence for a proinflammatory endothelial phenotype. The EPC-derived EC maintained this endothelial phenotype when expanded in roller bottles and subsequently seeded on polyglycolic acid-poly-L-lactic acid (PGA-PLLA) scaffolds, but microvessel formation was not observed. In contrast, EPC-derived EC seeded with human smooth muscle cells formed capillary-like structures throughout the scaffold (76.5 ± 35 microvessels/mm2). These results indicate that 1) EPC-derived EC can be expanded in vitro and seeded on biodegradable scaffolds with preservation of endothelial phenotype and 2) EPC-derived EC seeded with human smooth muscle cells form microvessels on porous PGA-PLLA scaffolds. These properties indicate that EPC may be well suited for creating microvascular networks within tissue-engineered constructs.
blood vessels; tissue engineering; polyglycolic acid-poly-L-lactic acid
This article has been cited by other articles:
|
|
 |
|
  Z. Gong and L. E. Niklason Small-diameter human vessel wall engineered from bone marrow-derived mesenchymal stem cells (hMSCs) FASEB J, June 1, 2008; 22(6): 1635 - 1648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. E. Isakson, A. K. Best, and B. R. Duling Incidence of protein on actin bridges between endothelium and smooth muscle in arterioles demonstrates heterogeneous connexin expression and phosphorylation Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2898 - H2904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Mayer Tissue Engineering for Cardiac Valve Surgery Card. Surg. Adult, January 1, 2008; 3(2008): 1649 - 1656. [Full Text]
|
|
|
|
 |
|
 X. Wu, M. W. Lensch, J. Wylie-Sears, G. Q. Daley, and J. Bischoff Hemogenic Endothelial Progenitor Cells Isolated from Human Umbilical Cord Blood Stem Cells, November 1, 2007; 25(11): 2770 - 2776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Melero-Martin, Z. A. Khan, A. Picard, X. Wu, S. Paruchuri, and J. Bischoff In vivo vasculogenic potential of human blood-derived endothelial progenitor cells Blood, June 1, 2007; 109(11): 4761 - 4768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Schatteman, M. Dunnwald, and C. Jiao Biology of bone marrow-derived endothelial cell precursors Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H1 - H18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Shepherd, D. R. Enis, F. Wang, Y. Suarez, J. S. Pober, and J. S. Schechner Vascularization and engraftment of a human skin substitute using circulating progenitor cell-derived endothelial cells FASEB J, August 1, 2006; 20(10): 1739 - 1741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. A. Khan, J. M. Melero-Martin, X. Wu, S. Paruchuri, E. Boscolo, J. B. Mulliken, and J. Bischoff Endothelial progenitor cells from infantile hemangioma and umbilical cord blood display unique cellular responses to endostatin Blood, August 1, 2006; 108(3): 915 - 921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Patel, B. Fine, M. Sandig, and K. Mequanint Elastin biosynthesis: The missing link in tissue-engineered blood vessels Cardiovasc Res, July 1, 2006; 71(1): 40 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. POLAK and A. E. BISHOP Stem Cells and Tissue Engineering: Past, Present, and Future Ann. N.Y. Acad. Sci., April 1, 2006; 1068(1): 352 - 366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E.J. Reinders, T. J. Rabelink, and D. M. Briscoe Angiogenesis and Endothelial Cell Repair in Renal Disease and Allograft Rejection J. Am. Soc. Nephrol., April 1, 2006; 17(4): 932 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. W.H. Sutherland, T. E. Perry, Y. Yu, M. C. Sherwood, E. Rabkin, Y. Masuda, G. A. Garcia, D. L. McLellan, G. C. Engelmayr Jr, M. S. Sacks, et al. From Stem Cells to Viable Autologous Semilunar Heart Valve Circulation, May 31, 2005; 111(21): 2783 - 2791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. March and B. H. Johnstone Cellular approaches to tissue repair in cardiovascular disease: the more we know, the more there is to learn Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H458 - H463. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
