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Activation of p38 MAPK and increased glucose transport in chronic hibernating swine myocardium

¹Division of Cardiology, Veterans Affairs Medical Center, and ²Division of Cardiology, University of Minnesota, Minneapolis, Minnesota 55417

Submitted 12 December 2003 ; accepted in final form 23 April 2004

In preconditioned myocardium, activation of the mitogen-activated protein kinase (MAPK) p38 leads to increased glucose uptake via enhanced GLUT-4 translocation. Glucose uptake is also increased in chronic hibernating myocardium, but the role of p38 MAPK and GLUT-4 translocation has not been studied. Nine swine underwent instrumentation of the proximal left anterior descending coronary artery (LAD) with a small, external constrictor. At 3 mo after instrumentation, myocardial glucose uptake by PET imaging was higher in the LAD than in the remote region under basal, fasted conditions (0.08 ± 0.02 vs. 0.04 ± 0.01 µmol·min^–1·g^–1, P < 0.05). Compared with the remote region, the LAD region demonstrated increased membrane-bound GLUT-4 relative to total content (61 ± 04 vs. 45 ± 06%, P < 0.05), higher glycogen (28.37 ± 4.41 vs. 19.26 ± 1.87 mg/g wet wt, P < 0.05), and increased inducible nitric oxide synthase (NOS) activity (1.43 ± 0.34 vs. 0.51 ± 0.21 activity/mg protein, P < 0.05). p38 MAPK was 47 ± 14% higher in the LAD than in the remote region (P < 0.05) and correlated well with the absolute degree of GLUT-4 membrane-bound translocation (r = 0.81, P < 0.01), relative increase in glycogen (r = 0.70, P < 0.05), and total NOS activity (r = 0.68, P < 0.05). In chronic hibernating myocardial tissue, p38 MAPK activation is increased under basal fasted conditions and correlates well with the increased degree of GLUT-4 translocation, glycogen accumulation, and NOS activity. As in preconditioned myocardium, activation of p38 MAPK may play an important role in the metabolic adaptations that characterize chronic hibernating myocardium.

GLUT-4; glucose uptake; preconditioned myocardium; inducible nitric oxide synthase

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