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This Article Full Text Full Text (PDF) All Versions of this Article: 287/5/H2234    most recent 00333.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Johnson, L. L. Articles by Williams, D. O. Search for Related Content PubMed PubMed Citation Articles by Johnson, L. L. Articles by Williams, D. O.

Hyperbaric oxygen solution infused into the anterior interventricular vein at reperfusion reduces infarct size in swine

¹Division of Cardiology, Rhode Island Hospital, Brown University, Providence, Rhode Island 02903; and ²TherOx Incorporated, Irvine, California 92612

Submitted 1 May 2003 ; accepted in final form 1 May 2004

This study was designed to test the hypothesis that raising myocardial O₂ via diffusion of a hyperbaric oxygen solution (AO) administered through the anterior interventricular vein (AIV) will reduce infarct size by reducing reperfusion injury associated with reduced neutrophil activation. In three pilot open-chest swine experiments, myocardial tissue PO₂ was monitored using an oxygen probe during coronary occlusion (Occl) and reperfusion (Rep). One control experiment had no AIV infusion; a second control received arterial blood drawn from the femoral artery infused into the AIV during Rep. In a third open-chest experiment, AO mixed with arterial blood was infused through the AIV at Rep. In controls, tissue PO₂ in the risk region (RR) rose early in Rep and then fell to Occl levels, whereas in AO-treated animals, myocardial PO₂ remained above baseline. The following three groups of five swine then underwent 60 min of left anterior descending coronary artery Occl and Rep: 1) arterial blood infused at Rep as controls (Con), 2) AO infused beginning 30 min after Rep (AO 30 min), and 3) AO infused immediately at Rep (AO 0 min). There were no differences among the three groups in hemodynamics or myocardial blood flow during baseline (BL) or Occl or in RR size. However, endocardial blood flow was significantly higher in RR during Rep in AO 0 min vs. control and AO 30 min (P = 0.01). Both infarct size (IS) as %heart and IS as %RR were lower in AO 0 min compared with Con and AO 30 min (P < 0.01 for both), and myeloperoxidase values were lower for epicardial (P < 0.001), midmyocardial (P = 0.03), and endocardial (P < 0.001) layers in AO 0 min. AO infused into the AIV immediately at Rep diffuses into the RR and reduces IS by reducing Rep injury associated with neutrophil activation.

infarction; ischemia