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This Article Full Text Full Text (PDF) All Versions of this Article: 287/6/H2634    most recent 00380.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Nygren, A. Articles by Giles, W. R. Search for Related Content PubMed PubMed Citation Articles by Nygren, A. Articles by Giles, W. R.

Heterogeneity of action potential durations in isolated mouse left and right atria recorded using voltage-sensitive dye mapping

¹Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada T2N 4N1; and ²Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, California 92093-0412

Submitted 26 April 2004 ; accepted in final form 15 July 2004

An imaging system for di-4-ANEPPS (4-[-[2-(di-n-butylamino)-6-naphthylvinyl]pyridinium]) voltage-sensitive dye recordings has been adapted for recording from an in vitro mouse heart preparation that consists of both atria in isolation. This approach has been used to study inter- and intra-atrial activation and conduction and to monitor action potential durations (APDs) in the left and right atrium. The findings from this study confirm some of our previous findings in isolated mouse atrial myocytes and demonstrate that many electrophysiological properties of mouse atria closely resemble those of larger mammals. Specifically, we made the following observations: 1) Activation in mouse atria originates in the sinoatrial node and spreads into the right atrium and, after a delay, into the left atrium. 2) APD in the left atrium is shorter than in the right atrium. 3) Sites in the posterior walls have longer APDs than sites in the atrial appendages. 4) Superfusion of this preparation with 4-aminopyridine and tetraethylammonium resulted in increases in APD, consistent with their inhibitory effects on the K⁺ currents known to be expressed in mouse atria. 5) The muscarinic agonist carbachol shortened APD in all areas of the preparation, except the left atrial appendage, in which carbachol had no statistically significant effect on APD. These results validate a new approach for monitoring activation, conduction, and repolarization in mouse atria and demonstrate that the physiological and pharmacological properties of mouse atria are sufficiently similar to those of larger animals to warrant further studies using this preparation.

optical mapping; potassium currents

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