| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Division of Pediatric Surgery, 2Department of Anesthesiology, 3Department of Biophysics, 4Department of Pharmacology and Toxicology, 5Division of Cardiothoracic Surgery, 6Department of Biochemistry, and 7Free Radical Research Center, Medical College of Wisconsin; and 8Section of Cardiothoracic Surgery, Children's Hospital of Wisconsin, Milwaukee, Wisconsin
Submitted 25 May 2004 ; accepted in final form 7 September 2004
We determined whether isoflurane can confer delayed cardioprotection in the adult rat by triggering increased production of reactive oxygen (ROS) and nitrogen species (RNS). Our objectives were to determine 1) the concentration of isoflurane that confers delayed cardioprotection in the adult rat, 2) the role of ROS and RNS in the induction of delayed cardioprotection, and 3) the cellular sources of ROS and RNS responsible for induction of delayed cardioprotection by isoflurane. Male Sprague-Dawley rats at 8 wk of age (n = 8 rats/group) were exposed to 0.5%, 0.8%, 1%, and 2% (vol/vol) isoflurane-100% oxygen for 2 h. Isoflurane conferred delayed cardioprotection 24 h later at a concentration of 0.8% (vol/vol). Administration of manganese (III) tetrakis (4-benzoic acid)porphyrin chloride (MnTBAP), a superoxide scavenger (15 mg/kg ip), or NG-nitro-L-arginine methyl ester (L-NAME), a general nitric oxide synthase inhibitor (15 mg/kg ip), 15 min before isoflurane treatment abolished the delayed cardioprotective effects of isoflurane. MnTBAP and L-NAME had no effect on delayed cardioprotection in untreated hearts. Perfusion of isolated hearts with hydroethidine, a fluorescent probe for superoxide, after isoflurane treatment resulted in a twofold increase in ethidine staining of isoflurane-treated hearts compared with untreated controls, which was attenuated by myxothiazol, an inhibitor of the mitochondrial electron transport chain (0.2 mg/kg ip) and L-NAME (15 mg/kg ip). Nitrite and nitrate content in isoflurane-treated hearts was 1.5-fold higher than in untreated hearts, whereas myocardial reduced glutathione levels were decreased by 13% in 0.8% but not in 1.0% isoflurane-treated hearts. We conclude that isoflurane confers delayed cardioprotection in the adult rat, triggered by ROS and RNS.
ischemia; nitric oxide synthase; nitric oxide; superoxide
This article has been cited by other articles:
|
|
 |
|
  J. Feng, E. Lucchinetti, G. Fischer, M. Zhu, K. Zaugg, M. C. Schaub, and M. Zaugg Cardiac remodelling hinders activation of cyclooxygenase-2, diminishing protection by delayed pharmacological preconditioning: role of HIF1{alpha} and CREB Cardiovasc Res, April 1, 2008; 78(1): 98 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Liang, Q. Wang, Y. Li, B. Kang, M. F. Eckenhoff, R. G. Eckenhoff, and H. Wei A Presenilin-1 Mutation Renders Neurons Vulnerable to Isoflurane Toxicity Anesth. Analg., February 1, 2008; 106(2): 492 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lucchinetti, J. Aguirre, J. Feng, M. Zhu, M. Suter, D. R. Spahn, L. Harter, and M. Zaugg Molecular Evidence of Late Preconditioning After Sevoflurane Inhalation in Healthy Volunteers Anesth. Analg., September 1, 2007; 105(3): 629 - 640. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Nilakantan, X. Zhou, G. Hilton, Y. Shi, J. E. Baker, A. K. Khanna, and G. M. Pieper Antagonizing reactive oxygen by treatment with a manganese (III) metalloporphyrin-based superoxide dismutase mimetic in cardiac transplants J. Thorac. Cardiovasc. Surg., April 1, 2006; 131(4): 898 - 906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wakeno-Takahashi, H. Otani, S. Nakao, H. Imamura, and K. Shingu Isoflurane induces second window of preconditioning through upregulation of inducible nitric oxide synthase in rat heart Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2585 - H2591. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
