| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
New York Harbor Veterans Affairs Healthcare System and Downstate Medical Center, State University of New York, Brooklyn, New York
Submitted 28 May 2004 ; accepted in final form 19 August 2004
Repolarization alternans has been considered a strong marker of electrical instability. The objective of this study was to investigate the hypothesis that ischemia-induced contrasting effects on the kinetics of membrane voltage and intracellular calcium transient (CaiT) can explain the vulnerability of the ischemic heart to repolarization alternans. Ischemia-induced changes in action potential (AP) and CaiT resulting in alternans were investigated in perfused Langendorff guinea pig hearts subjected to 10–15 min of global no-flow ischemia followed by 10–15 min of reperfusion. The heart was stained with 100 µl of rhod-2 AM and 25 µl of RH-237, and AP and CaiT were simultaneously recorded with an optical mapping system of two 16 x 16 photodiode arrays. Ischemia was associated with shortening of AP duration (D) but delayed upstroke, broadening of peak, and slowed decay of CaiT resulting in a significant increase of CaiT-D. The changes in APD were spatially heterogeneous in contrast to a more spatially homogeneous lengthening of CaiT-D. CaiT alternans could be consistently induced with the introduction of a shorter cycle when the upstroke of the AP occurred before complete relaxation of the previous CaiT and generated a reduced CaiT. However, alternans of CaiT was not necessarily associated with alternans of APD, and this was correlated with the degree of spatially heterogeneous shortening of APD. Sites with less shortening of APD developed alternans of both CaiT and APD, whereas sites with greater shortening of APD could develop a similar degree of CaiT alternans but slight or no APD alternans. This resulted in significant spatial dispersion of APD. The study shows that the contrasting effects of ischemia on the duration of AP and CaiT and, in particular, on their spatial distribution explain the vulnerability of ischemic heart to alternans and the increased dispersion of repolarization during alternans.
electrophysiology; arrhythmias; optical mapping
This article has been cited by other articles:
|
|
 |
|
  S. Kapur, J. A. Wasserstrom, J. E. Kelly, A. H. Kadish, and G. L. Aistrup Acidosis and ischemia increase cellular Ca2+ transient alternans and repolarization alternans susceptibility in the intact rat heart Am J Physiol Heart Circ Physiol, May 1, 2009; 296(5): H1491 - H1512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tao, S. C. O'Neill, M. E. Diaz, Y. T. Li, D. A. Eisner, and H. Zhang Alternans of cardiac calcium cycling in a cluster of ryanodine receptors: a simulation study Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H598 - H609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kay, L. Swift, B. Martell, A. Arutunyan, and N. Sarvazyan Locations of ectopic beats coincide with spatial gradients of NADH in a regional model of low-flow reperfusion Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2400 - H2405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. T. Clusin Mechanisms of calcium transient and action potential alternans in cardiac cells and tissues Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H1 - H10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Cordeiro, J. E. Malone, J. M. Di Diego, F. S. Scornik, G. L. Aistrup, C. Antzelevitch, and J. A. Wasserstrom Cellular and subcellular alternans in the canine left ventricle Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3506 - H3516. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
