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Estrogen-induced contraction of coronary arteries is mediated by superoxide generated in vascular smooth muscle

¹Department of Pharmacology and Toxicology, ²Vascular Biology Center, and ³Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia

Submitted 22 November 2004 ; accepted in final form 21 May 2005

Although previous studies demonstrated beneficial effects of estrogen on cardiovascular function, the Women's Health Initiative has reported an increased incidence of coronary heart disease and stroke in postmenopausal women taking hormone replacement therapy. The objective of the present study was to identify a molecular mechanism whereby estrogen, a vasodilatory hormone, could possibly increase the risk of cardiovascular disease. Isometric contractile force recordings were performed on endothelium-denuded porcine coronary arteries, whereas molecular and fluorescence studies identified estrogen signaling molecules in coronary smooth muscle. Estrogen (1–1,000 nM) relaxed arteries in an endothelium-independent fashion; however, when arteries were pretreated with agents to uncouple nitric oxide (NO) production from NO synthase (NOS), estrogen contracted coronary arteries with an EC₅₀ of 7.3 ± 4 nM. Estrogen-induced contraction was attenuated by reducing superoxide (O₂^–). Estrogen-stimulated O₂^– production was detected in NOS-uncoupled coronary myocytes. Interestingly, only the type 1 neuronal NOS isoform (nNOS) was detected in myocytes, making this protein a likely target mediating both estrogen-induced relaxation and contraction of endothelium-denuded coronary arteries. Estrogen-induced contraction was completely inhibited by 1 µM nifedipine or 10 µM indomethacin, indicating involvement of dihydropyridine-sensitive calcium channels and contractile prostaglandins. We propose that a single molecular mechanism can mediate the dual and opposite effect of estrogen on coronary arteries: by stimulating type 1 nNOS in coronary arteries, estrogen produces either vasodilation via NO or vasoconstriction via O₂^–.

nitric oxide; coronary circulation

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