HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 290/4/H1642    most recent 00586.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Horton, J. W. Articles by White, D. J. Search for Related Content PubMed PubMed Citation Articles by Horton, J. W. Articles by White, D. J.

Hypertonic saline dextran after burn injury decreases inflammatory cytokine responses to subsequent pneumonia-related sepsis

Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas

Submitted 2 June 2005 ; accepted in final form 3 November 2005

The present study examined the hypothesis that hypertonic saline dextran (HSD), given after an initial insult, attenuates exaggerated inflammation that occurs with a second insult. Adult rats (n = 15 per group) were divided into groups 1 (sham burn), 2 [40% total body surface area burn + 4 ml/kg isotonic saline (IS) + 4 ml·kg^–1·% burn^–1 lactated Ringer solution (LR)], and 3 (burn + 4 ml/kg HSD + LR), all studied 24 h after burns. Groups 4 (sham burn), 5 (burn + IS + LR), and 6 (burns + HSD + LR) received intratracheal (IT) vehicle 7 days after burns; groups 7 (burn + IS + LR) and 8 (burn + HSD + LR) received IT Streptococcus pneumoniae (4 x 10⁶ colony-forming units) 7 days after burn. Groups 4–8 were studied 8 days after burn and 24 h after IT septic challenge. When compared with sham burn, contractile defects occurred 24 h after burn in IS-treated but not HSD-treated burns. Cardiac inflammatory responses (pg/ml TNF-) were evident with IS (170 ± 10) but not HSD (45 ± 5) treatment vs. sham treatment (80 ± 15). Pneumonia-related sepsis 8 days after IS-treated burns (group 7) exacerbated TNF- responses/contractile dysfunction vs. IS-treated burns in the absence of sepsis (P < 0.05). Sepsis that occurred after HSD-treated burns (group 8) had less myocyte TNF- secretion/better contractile function than IS-treated burns given septic challenge (group 7, P < 0.05). We conclude that an initial burn injury exacerbates myocardial inflammation/dysfunction occurring with a second insult; giving HSD after the initial insult attenuates myocardial inflammation/dysfunction associated with a second hit, suggesting that HSD reduces postinjury risk for infectious complications.

rat model; Langendorff perfusion; burn complicated by sepsis; cardiac myocytes; tumor necrosis factor-; interleukin-1; interleukin-6

This article has been cited by other articles:

				M. Z. Chen, X. Zhu, H.-Q. Sun, Y. S. Mao, Y. Wei, M. Yamamoto, and H. L. Yin Oxidative Stress Decreases Phosphatidylinositol 4,5-Bisphosphate Levels by Deactivating Phosphatidylinositol- 4-phosphate 5-Kinase {beta} in a Syk-dependent Manner J. Biol. Chem., August 28, 2009; 284(35): 23743 - 23753. [Abstract] [Full Text] [PDF]

				H. Zhang, H.-Y. Wang, R. Bassel-Duby, D. L. Maass, W. E. Johnston, J. W. Horton, and W. Tao Role of interleukin-6 in cardiac inflammation and dysfunction after burn complicated by sepsis Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2408 - H2416. [Abstract] [Full Text] [PDF]