HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 290/5/H2108    most recent 00395.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Borge, B. A. Articles by Reed, R. K. Search for Related Content PubMed PubMed Citation Articles by Borge, B. A. Articles by Reed, R. K.

Changes in plasma protein extravasation in rat skin during inflammatory challenges evaluated by microdialysis

Department of Biomedicine, Section of Physiology, University of Bergen, Bergen, Norway

Submitted 21 April 2005 ; accepted in final form 15 December 2005

Docetaxel and prostaglandin E₁ (PGE₁) increase transcapillary albumin extravasation and reduce interstitial fluid pressure in the skin. In this study the microdialysate concentration (C_m) of ¹²⁵I-labeled human serum albumin (¹²⁵I-HSA) and different-sized endogenous plasma proteins (EPP) was compared to evaluate changes in transcapillary extravasation of plasma proteins. ¹²⁵I-HSA was also used to estimate changes in the specific activity of albumin. Extravasation of ¹²⁵I-HSA and EPP from plasma to interstitium in the rat skin was compared during continuous administration of docetaxel and PGE₁ by using microdialysis in anesthetized rats. Also, 20 ml of Ringer solution (RS) were injected intravenously during 10 min in a separate group. Two hollow plasmapheresis fibers (3 cm, cut off 3,000 kDa), one acting as control, were placed subcutaneously on the back skin and perfused with RS (5 µl/min, 140 min, collected every 10 min). The size of the different EPP was estimated to be 73, 65, 56, 47, and 39 Å, separated by a size-exclusion high-performance liquid chromatography column and quantified by UV detection (280 nm). Docetaxel (0.5 mg/ml, n = 5) increased C_m of ¹²⁵I-HSA and EPP of sizes 73, 65, 56, and 39 Å significantly (P < 0.05) compared with control. PGE₁ (20 µg/ml, n = 6) increased C_m of ¹²⁵I-HSA significantly (P < 0.05) but none of the different-sized EPP was increased compared with control. Intravenous RS (20 ml, n = 6) increased C_m of ¹²⁵I-HSA and increased all the different-sized EPP significantly (P < 0.05) compared with control. Although the microdialysis method is able to monitor qualitative changes in capillary permeability, a quantitative determination of the capillary reflection coefficient or permeability-surface area product was not possible, because steady state between plasma and dialysate was not achieved during the measurement period. The different pattern of extravasation of EPP and ¹²⁵I-HSA after docetaxel, PGE₁, and RS indicates increased interstitial transport rate and/or increased capillary permeability after docetaxel and RS, whereas PGE₁ seems to increase transcapillary fluid flux without altering the permeability.

high-performance liquid chromatography; prostaglandin E₁; docetaxel; transcapillary transport